Dr. Christine Beuck

Kontakt

  • +49 (0)201 183-2929
  • E-Mail
  • S03 S00 A35

 

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Forschung

Nuclear Magnetic Resonance (NMR) spectroscopy

NMR spectroscopy is a versatile tool to study proteins and their molecular interactions. As part of the CRC 1093, I investigate both the interactions of proteins with their natural binding partners and synthetic ligands designed to target protein surfaces. NMR is a powerful method that can directly determine the protein residues involved in interactions with other biomolecules or synthetic ligands. I established the use of side chain specific NMR experiments for lysine and arginine residues to precisely monitor supramolecular ligand binding, which, in contrast to the standard amide 15N-HSQC experiment, allows to rank multiple binding sites within one protein. For larger proteins, fast-acquisition (BEST-TROSY; SOFAST) as well as Methyl-TROSY experiments are employed. Furthermore, NMR is used to determine the structures of proteins or isolated domains and probe their size, oligomerization status and dynamics or reaction kinetics.

Protein Structure Determination

I elucidate the structures of proteins with both solution NMR spectroscopy and X-Ray crystallography.

NMR structures:

X-Ray structures:

Biochemical Characterization of Protein Complexes

To perform their task within the cell, proteins are interacting with a multitude of other biomolecules like other proteins, nu

Curriculum Vitae

Professional career

Seit 2014 Assistenzprofessorin(Habilitandin) bei Prof. Peter Bayer, Institut für Strukturelle und Medizinische Biochemie
2012 - 2014 Postdoktorandin und wissenschaftliche Mitarbeiterin, bei Prof. Dr. J. R. Williamson, The Scripps Research Institute, La Jolla, USA
2005 Gastwissenschaftlerin bei Prof. Dr. M. Shionoya, Tokyo University, Tokyo, Japan, im Rahmen des GDCh/DFG Japan Austauschprogramms 2005
2003 Gastwissenschaftlerin bei Dr. habil. S. Klimašauskas, Institute of Biotechnology, Vilnius, Lithuania

 

Wissenschaftliche Ausbildung

2001 - 2005 Promotion in Chemie bei Prof. Dr. E. Weinhold, RWTH Aachen
1996 - 2001 Chemiestudium (Dipl.) an der Technischen Universität Dortmund, Diplomarbeit bei Prof. Dr. E. Weinhold, Max Planck Institut für Molekulare Physiologie

 

 

Publikationen

    Artikel in Zeitschriften

  • Hommel, Katrin; Kauth, Alisa-Maite A.; Kirupakaran, Abbna; Theisen, Sebastian; Hayduk, Matthias; Niemeyer, Felix C.; Beuck, Christine; Zadmard, Reza; Bayer, Peter; Jan Ravoo, Bart; Voskuhl, Jens; Schrader, Thomas; Knauer, Shirley K.
    Functional Linkers Support Targeting of Multivalent Tweezers to Taspase1
    In: Chemistry - A European Journal Jg. 30 (2024) Nr. 56, e202401542
    Online Volltext: dx.doi.org/ (Open Access)
  • Schillinger, Jasmin; Koci, Michelle; Bravo-Rodriguez, Kenny; Heilmann, Geronimo; Kaschani, Farnusch; Kaiser, Markus; Beuck, Christine; Luecke, Hartmut; Huber, Robert; Hellerschmied-Jelinek, Doris; Burston, Steven G.; Ehrmann, Michael
    High resolution analysis of proteolytic substrate processing
    In: The Journal of Biological Chemistry (JBC) Jg. 300 (2024) Nr. 11, 107812
    Online Volltext: dx.doi.org/ (Open Access)
  • Wolff, Natalie; Białas, Nataniel; Loza, Kateryna; Heggen, Marc; Schaller, Torsten; Niemeyer, Felix; Weidenthaler, Claudia; Beuck, Christine; Bayer, Peter; Prymak, Oleg; Oliveira, Cristiano L. P.; Epple, Matthias
    Increased Cytotoxicity of Bimetallic Ultrasmall Silver–Platinum Nanoparticles (2 nm) on Cells and Bacteria in Comparison to Silver Nanoparticles of the Same Size
    In: Materials Jg. 17 (2024) Nr. 15, 3702
    Online Volltext: dx.doi.org/ (Open Access)
  • Wolff, Natalie; Beuck, Christine; Schaller, Torsten; Epple, Matthias
    Possibilities and limitations of solution-state NMR spectroscopy to analyze the ligand shell of ultrasmall metal nanoparticles
    In: Nanoscale Advances Jg. 6 (2024) Nr. 13, S. 3285 - 3298
    Online Volltext: dx.doi.org/ (Open Access)
  • Beaufort, Nathalie; Ingendahl, Linda; Merdanovic, Melisa; Schmidt, Andree; Podlesainski, David; Richter, Tim; Neumann, Thorben; Kuszner, Michael; Vetter, Ingrid R.; Stege, Patricia; Burston, Steven G.; Filipovic, Anto; Ruiz-Blanco, Yasser B.; Bravo Rodriguez, Kenny; Mieres-Perez, Joel; Beuck, Christine; Uebel, Stephan; Zobawa, Monika; Schillinger, Jasmin; Malik, Rainer; Todorov-Völgyi, Katalin; Rey, Juliana; Roberti, Annabell; Hagemeier, Birte; Wefers, Benedikt; Müller, Stephan A.; Wurst, Wolfgang; Sanchez-Garcia, Elsa; Zimmermann, Alexander; Hu, Xiao-Yu; Clausen, Tim; Huber, Robert; Lichtenthaler, Stefan F.; Schmuck, Carsten; Giese, Michael; Kaiser, Markus; Ehrmann, Michael; Dichgans, Martin
    Rational correction of pathogenic conformational defects in HTRA1
    In: Nature Communications Jg. 15 (2024) Nr. 1, 5944
    Online Volltext: dx.doi.org/ (Open Access)
  • Wagner, Lisa-Sofie; Prymak, Oleg; Schaller, Torsten; Beuck, Christine; Loza, Kateryna; Niemeyer, Felix; Gumbiowski, Nina; Kostka, Kathrin; Bayer, Peter; Heggen, Marc; Oliveira, Cristiano L. P.; Epple, Matthias
    The Molecular Footprint of Peptides on the Surface of Ultrasmall Gold Nanoparticles (2 nm) Is Governed by Steric Demand
    In: Journal of Physical Chemistry B: Condensed Matter, Materials, Surfaces, Interfaces & Biophysical Chemistry Jg. 128 (2024) Nr. 17, S. 4266 - 4281
    Online Volltext: dx.doi.org/
  • Neblik, Jonas; Kirupakaran, Abbna; Beuck, Christine; Mieres-Perez, Joel; Niemeyer, Felix; Le, My-Hue; Telgheder, Ursula; Schmuck, Jessica Felice; Dudziak, Alexander; Bayer, Peter; Sanchez-Garcia, Elsa; Westermann, Stefan; Schrader, Thomas
    Multivalent Molecular Tweezers Disrupt the Essential NDC80 Interaction with Microtubules
    In: Journal of the American Chemical Society: JACS Jg. 145 (2023) Nr. 28, S. 15251 - 15264
    Online Volltext: dx.doi.org/
  • Seiler, Theresa; Lennartz, Annika; Klein, Kai; Hommel, Katrin; Figueroa Bietti, Antonio; Hadrovic, Inesa; Kollenda, Sebastian; Sager, Jonas; Beuck, Christine; Chlosta, Emilia; Bayer, Peter; Juul-Madsen, Kristian; Vorup-Jensen, Thomas; Schrader, Thomas; Epple, Matthias; Knauer, Shirley; Hartmann, Laura
    Potentiating Tweezer Affinity to a Protein Interface with Sequence-Defined Macromolecules on Nanoparticles
    In: Biomacromolecules Jg. 24 (2023) Nr. 8, S. 3666 - 3679
    Online Volltext: dx.doi.org/
  • Blüggel, Mike; Kröning, Alexander; Kracht, Matthias; van den Boom, Johannes; Dabisch, Matthias; Göhring, Anna; Kaschani, Farnusch; Kaiser, Markus; Bayer, Peter; Meyer, Hemmo; Beuck, Christine
    The UBX domain in UBXD1 organizes ubiquitin binding at the C-terminus of the VCP/p97 AAA-ATPase
    In: Nature Communications Jg. 14 (2023) Nr. 1, 3258
    Online Volltext: dx.doi.org/ Online Volltext (Open Access)
  • Wolff, Natalie; Loza, Kateryna; Heggen, Marc; Schaller, Torsten; Niemeyer, Felix; Bayer, Peter; Beuck, Christine; Oliveira, Cristiano L P; Prymak, Oleg; Weidenthaler, Claudia; Epple, Matthias
    Ultrastructure and Surface Composition of Glutathione-Terminated Ultrasmall Silver, Gold, Platinum, and Alloyed Silver-Platinum Nanoparticles (2 nm)
    In: Inorganic Chemistry Jg. 62 (2023) Nr. 42, S. 17470 - 17485
    Online Volltext: dx.doi.org/
  • Höing, Alexander; Struth, Robin; Beuck, Christine; Rafieiolhosseini, Neda; Hoffmann, Daniel; Stauber, Roland H.; Bayer, Peter; Niemeyer, Jochen; Knauer, Shirley
    Dual activity inhibition of threonine aspartase 1 by a single bisphosphate ligand
    In: RSC Advances Jg. 12 (2022) Nr. 53, S. 34176 - 34184
    Online Volltext: dx.doi.org/ (Open Access)
  • Höing, Alexander; Kirupakaran, Abbna; Beuck, Christine; Pörschke, Marius; Niemeyer, Felix; Seiler, Theresa; Hartmann, Laura; Bayer, Peter; Schrader, Thomas; Knauer, Shirley
    Recognition of a Flexible Protein Loop in Taspase 1 by Multivalent Supramolecular Tweezers
    In: Biomacromolecules Jg. 23 (2022) Nr. 11, S. 4504 - 4518
    Online Volltext: dx.doi.org/
  • Wetzel, Oliver; Prymak, Oleg; Loza, Kateryna; Gumbiowski, Nina; Heggen, Marc; Bayer, Peter; Beuck, Christine; Weidenthaler, Claudia; Epple, Matthias
    Water-Based Synthesis of Ultrasmall Nanoparticles of Platinum Group Metal Oxides (1.8 nm)
    In: Inorganic Chemistry Jg. 61 (2022) Nr. 12, S. 5133 - 5147
    Online Volltext: dx.doi.org/
  • Wetzel, Oliver; Hosseini, Shabnam; Loza, Kateryna; Heggen, Marc; Prymak, Oleg; Bayer, Peter; Beuck, Christine; Schaller, Torsten; Niemeyer, Felix; Weidenthaler, Claudia; Epple, Matthias
    Metal-Ligand Interface and Internal Structure of Ultrasmall Silver Nanoparticles (2 nm)
    In: Journal of Physical Chemistry B: Condensed Matter, Materials, Surfaces, Interfaces & Biophysical Chemistry Jg. 125 (2021) Nr. 21, S. 5645 - 5659
    Online Volltext: dx.doi.org/
  • van der Meer, Selina Beatrice; Hadrovic, Inesa; Meiners, Annika; Loza, Kateryna; Heggen, Marc; Knauer, Shirley; Bayer, Peter; Schrader, Thomas; Beuck, Christine; Epple, Matthias
    New tools to probe the protein surface : Ultrasmall gold nanoparticles carry amino acid binders
    In: Journal of Physical Chemistry B: Condensed Matter, Materials, Surfaces, Interfaces & Biophysical Chemistry Jg. 125 (2021) Nr. 1, S. 115 - 127
    Online Volltext: dx.doi.org/ (Open Access)
  • Ruks, Tatjana; Loza, Kateryna; Heggen, Marc; Prymak, Oleg; Sehnem, Andre Luiz; Oliveira, Cristiano L. P.; Bayer, Peter; Beuck, Christine; Epple, Matthias
    Peptide-Conjugated Ultrasmall Gold Nanoparticles (2 nm) for Selective Protein Targeting
    In: ACS Applied Bio Materials Jg. 4 (2021) Nr. 1, S. 945 - 965
    Online Volltext: dx.doi.org/ (Open Access)
  • Meiners, Annika; Bäcker, Sandra; Hadrovic, Inesa; Heid, Christian; Beuck, Christine; Ruiz-Blanco, Yasser B.; Mieres-Perez, Joel; Pörschke, Marius; Grad, Jean-Noël; Vallet, Cecilia; Hoffmann, Daniel; Bayer, Peter; Sánchez-García, Elsa; Schrader, Thomas; Knauer, Shirley
    Specific inhibition of the Survivin–CRM1 interaction by peptide-modified molecular tweezers
    In: Nature Communications Jg. 12 (2021) Nr. 1, S. 1505
    Online Volltext: dx.doi.org/ Online Volltext (Open Access)
  • Ruks, Tatjana; Loza, Kateryna; Heggen, Marc; Ottmann, Christian; Bayer, Peter; Beuck, Christine; Epple, Matthias
    Targeting the Surface of the Protein 14-3-3 by Ultrasmall (1.5 nm) Gold Nanoparticles Carrying the Specific Peptide CRaf
    In: ChemBioChem Jg. 22 (2021) Nr. 8, S. 1456 - 1463
    Online Volltext: dx.doi.org/ (Open Access)
  • Vallet, Cecilia; Aschmann, Dennis; Beuck, Christine; Killa, Matthias; Meiners, Annika; Mertel, Marcel; Ehlers, Martin; Bayer, Peter; Schmuck, Carsten; Giese, Michael R. A.; Knauer, Shirley
    Functional Disruption of the Cancer-Relevant Interaction between Survivin and Histone H3 with a Guanidiniocarbonyl Pyrrole Ligand
    In: Angewandte Chemie International Edition Jg. 59 (2020) Nr. 14, S. 5567 - 5571
    Online Volltext: dx.doi.org/ (Open Access)
  • Bayer, Peter; Matena, Anja; Beuck, Christine
    NMR Spectroscopy of supramolecular chemistry on protein surfaces
    In: Beilstein Journal of Organic Chemistry Jg. 16 (2020) S. 2505 - 2522
    Online Volltext: dx.doi.org/ (Open Access)
  • van der Meer, Selina; Loza, Kateryna; Wey, Karolin; Heggen, Marc; Beuck, Christine; Bayer, Peter; Epple, Matthias
    Click chemistry on the surface of ultrasmall gold nanoparticles (2 nm) for covalent ligand attachment followed by nmr spectroscopy
    In: Langmuir Jg. 35 (2019) Nr. 22, S. 7191 - 7204
    Online Volltext: dx.doi.org/
  • Bartsch, Lina; Bartel, Maria; Gigante, Alba; Iglesias-Fernández, Javier; Ruiz-Blanco, Yasser B.; Beuck, Christine; Briels, Jeroen; Tötsch, Niklas; Bayer, Peter; Sánchez-García, Elsa; Ottmann, Christian; Schmuck, Carsten
    Multivalent Ligands with Tailor-Made Anion Binding Motif as Stabilizers of Protein–Protein Interactions
    In: ChemBioChem Jg. 20 (2019) Nr. 23, S. 2921 - 2926
    Online Volltext: dx.doi.org/
  • Ruks, Tatjana; Beuck, Christine; Schaller, Torsten; Niemeyer, Felix; Zähres, Manfred; Loza, Kateryna; Heggen, Marc; Hagemann, Ulrich; Mayer, Christian; Bayer, Peter; Epple, Matthias
    Solution NMR Spectroscopy with Isotope-Labeled Cysteine (¹³C and ¹⁵N) Reveals the Surface Structure of l -Cysteine-Coated Ultrasmall Gold Nanoparticles (1.8 nm)
    In: Langmuir Jg. 35 (2019) Nr. 3, S. 767 - 778
    Online Volltext: dx.doi.org/
  • Blüggel, Mike; van den Boom, Johannes; Meyer, Hemmo; Bayer, Peter; Beuck, Christine
    Structure of the PUB Domain from Ubiquitin Regulatory X Domain Protein 1 (UBXD1) and Its Interaction with the p97 AAA+ ATPase
    In: Biomolecules Jg. 9 (2019) Nr. 12, 876
    Online Volltext: dx.doi.org/ Online Volltext (Open Access)
  • Hogeweg, Anna; Sowislok, Andrea; Schrader, Thomas; Beuck, Christine
    An NMR Method To Pinpoint Supramolecular Ligand Binding to Basic Residues on Proteins
    In: Angewandte Chemie International Edition Jg. 56 (2017) Nr. 46, S. 14758 - 14762
    Online Volltext: dx.doi.org/
  • Trusch, Franziska; Kowski, Klaus; Bravo-Rodriguez, Kenny; Beuck, Christine; Sowislok, Andrea; Wettig, Burkhard; Matena, Anja; Sánchez-García, Elsa; Meyer, Hemmo; Schrader, Thomas; Bayer, Peter
    Molecular tweezers target a protein-protein interface and thereby modulate complex formation
    In: Chemical Communications: ChemComm Jg. 52 (2016) Nr. 98, S. 14141 - 14144
    Online Volltext: dx.doi.org/ (Open Access)
  • van den Boom, Johannes; Trusch, Franziska; Hoppstock, Lukas; Beuck, Christine; Bayer, Peter
    Structural Characterization of the Loop at the Alpha-Subunit C-Terminus of the Mixed Lineage Leukemia Protein Activating Protease Taspase1
    In: PLoS ONE Jg. 11 (2016) Nr. 3, S. e0151431
    Online Volltext: dx.doi.org/ Online Volltext (Open Access)
  • Beuck, Christine; Williamson, James R.; Wüthrich, Kurt; Serrano, Pedro
    The acidic domain is a unique structural feature of the splicing factor SYNCRIP
    In: Protein Science Jg. 25 (2016) Nr. 8, S. 1545 - 1550
    Online Volltext: dx.doi.org/ (Open Access)
  • Beuck, Christine; Weinhold, Elmar
    Reversibly locked thionucleobase pairs in DNA to study base flipping enzymes
    In: Beilstein Journal of Organic Chemistry Jg. 10 (2014) S. 2293 - 2306
    Online Volltext: dx.doi.org/ (Open Access)