E. coli Proteases

The E. coli K12 genome encodes at least 74 peptidases/proteases.

Proteases were classified according to their celluar localisation.
View all proteases.

View lists of


Note that some proteases have not yet been classified. Several proteases are not seperately listed because there are only few family members. Also, proteases encoded on plasmids, phages or by pathogenic E. coli are not included in these lists.

All Proteases of E. coli

74 peptidases/proteases are listed. The majority are metalloproteases, followed by Ser proteases, Cys proteases and Asp proteases. Proteases are listed according to their celluar localisation and in alphabetical order. MWs of periplasmic and outer membrane proteases are given for the unprocessed precursor proteins. Clicking on the protein name will open the latest UniProt file.

The topology for each cytoplasmic membrane protein is predicted. Protein sequences are presented in lower case and the TMs in upper case.

Proteases localised in the

Cytoplasm

 

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Cytoplasmic membrane

 

  • Protease DegS (degS)
     

    Peptidase family S2C InterPro
    Function Essential serine protease, Cleaves RseA to induce sigma E cascade
    Structure PDB
    transmembrane domains: 1 (N-in)
    TMHMM gives 5-27
    SP gives signal seq.
     
    mfvkllrsVA IGLIVGAILL VAMPSLrsln plstpqfdst detpasynla vrraapavvn
    vynrglntns hnqleirtlg sgvimdqrgy iitnkhvind adqiivalqd grvfeallvg
    sdsltdlavl kinatgglpt ipinarrvph igdvvlaign pynlgqtitq giisatgrig
    lnptgrqnfl qtdasinhgn sggalvnslg elmgintlsf dksndgetpe gigfaipfql
    atkimdklir dgrvirgyig iggreiaplh aqgggidqlq givvnevspd gpaanagiqv
    ndliisvdnk paisaletmd qvaeirpgsv ipvvvmrddk qltlqvtiqe ypatn
    MW 37581 Da
    355 aa

     
  • FtsH (ftsH/hflB)
     

    Peptidase family M41/AAA Protease InterPro
    Function essential ATP-dependent zinc metalloprotease, cleaves soluble and integral membrane proteins
    Structure PDB
     
    transmembrane domains:2 (potential)

    TMHMM gives 5-24 97-119
    SP gives 5-24 96-120

    maknLILWLV IAVVLMSVFQ SFgpsesngr kvdystflqe vnndqvrear
    ingreinvtk kdsnryttyi pvqdpklldn lltknvkvvg eppeepSLLA
    SIFISWFPML LLIGVWIFFm rqmqggggkg amsfgkskar mltedqiktt
    fadvagcdea keevaelvey lrepsrfqkl ggkipkgvlm vgppgtgktl
    200
    lakaiageak vpfftisgsd fvemfvgvga srvrdmfeqa kkaapciifi
    deidavgrqr gaglggghde reqtlnqmlv emdgfegneg iiviaatnrp
    dvldpallrp grfdrqvvvg lpdvrgreqi lkvhmrrvpl apdidaaiia
    rgtpgfsgad lanlvneaal faargnkrvv smvefekakd kimmgaerrs
    400
    mvmteaqkes tayheaghai igrlvpehdp vhkvtiiprg ralgvtfflp
    egdaisasrq klesqistly ggrlaeeiiy gpehvstgas ndikvatnla
    rnmvtqwgfs eklgpllyae eegevflgrs vakakhmsde tariidqevk
    aliernynra rqlltdnmdi lhamkdalmk yetidapqid dlmarrdvrp
    600
    pagweepgas nnsgdngspk aprpvdeprt pnpgntmseq lgdk

     MW 70708 Da
    644 aa

     
  • GlpG (glpG)
     

    Peptidase family s54 InterPro
    Function  
    Structure transmembrane domains: 6 (potential)
     
    Phobius gives 96-115 135-160 172-188 194-212 224-242 248-267
    SP gives none
     
    mlmitsfanp rvaqafvdym atqgviltiq qhnqsdvwla desqaervra
    Elarflenpa dprylaaswq aghtgsglhy rrypffaalr eragpVTWVM
    MIACVVVFIA MQILGdqevm lwlawpfdpt lkfeFWRYFT HALMHFSLMH
    ILFNLLWWWY LGGAVekrlg sgkLIVITLI SALLSGYVQQ kfsgpWFGG
    SGVVYALMGY VWLrgerdpq sgIYLQRGLI IFALIWIVAG WFdlfGMSMA
    NGAHIAGLAV GLAMAFVdsl narkrk
    MW 31307 Da
    276 aa

     
  • Heat shock protein HtpX (htpX)
     

    Peptidase family M48 InterPro
    Function  
    Structure transmembrane domains: 4 (potential)
     
    TMHMM gives 5-27 32-54 158-180 195-217
    SP gives 7-27 35-51 158-178 193-213
     
    mmriALFLLT NLAVMVVFGL VLSLTGIqss sVQGLMIMAL LFGFGGSFVS
    LLMSkwmalr svggevieqp rnererwlvn tvatqarqag iampqvaiyh
    apdinafatg arrdaslvav stgllqnmsp deaeaviahe ishiangdmv
    tmtliqgVVN TFVIFISRIL AQLAAGFMGG nrdegeesng npliYFAVAT
    200
    VLELVFGILA SIITMWFsrh refhadagsa klvgrekmia alqrlktsye
    pqeatsmmal cingksksls elfmthppld kriealrtge ylk
    MW 31923 Da
    293 aa

     
  • Leader peptidase Lep4/Prepilin-like protein (hofD/hopD)
     

    Peptidase family A24 InterPro
    Function Leader peptidase, cleaves type-4 fimbrial leader sequence
    Structure transmembrane domains: 7 (potential), SP has 6
     
    TMHMM gives 7-29 49-71 78-100 104-122 127-149 169-191 204-223
    SP gives 3-23 68-88 104-124 128-148 175-195 203-223
     
    mtMLLPLFIL VGFIADYFVN AIAYhlsple dktaltfrqv lvhfrqkkYA
    WHDTVPLILC VAAAIACALA PFtpIVTGAL FLYFCFVLTL SVIdfrtqll
    pdkLTLPLLW LGLVFNAQYG LIdlhdaVYG AVAGYGVLWC VYWGVWLVCh
    keglgygdfk llaaagaWCG WQTLPMILLI ASLGGIGYAI Vsqllqrrti
    200
    ttIAFGPWLA LGSMINLGYL AWIsy
    MW 24957 Da
    225 aa

     
  • Leader peptidase I (LepB)
     

    Peptidase family s26 InterPro
    Function General leader peptidase
    Structure PDB
     

    transmembrane domains: 2 (N-out)
     
    TMHMM gives 5-27 60-82
    SP gives 4-22 59-77
     
    manmFALILV IATLVTGILW CVDKFFFapk rrerqaaaqa aagdsldkat
    lkkvapkpgW LETGASVFPV LAIVLIVRSF IYepfqipsg smmptlligd
    filvekfayg ikdpiyqktl ietghpkrgd ivvfkypedp kldyikravg
    lpgdkvtydp vskeltiqpg cssgqacena lpvtysnvep sdfvqtfsrr
    200
    nggeatsgff evpknetken girlserket lgdvthrilt vpiaqdqvgm
    yyqqpgqqla twivppgqyf mmgdnrdnsa dsrywgfvpe anlvgratai
    wmsfdkqege wptglrlsri ggih

    MW 35960 Da
    324 aa

     
  • Lipoprotein signa l peptidase (lspA)
     

    Peptidase family A8 InterPro
    Function Leader peptidase, removes signal peptides from lipoproteins
    Structure transmemebrane domains: 4 (potential)
     
    TMHMM gives 13-35 67-86 99-121 136-158
    SP gives 12-26 70-88 96-113 142-159
     
    msqsicstgl rwLWLVVVVL IIDLGSKYLI LQNFAlgdtv plfpslnlhy
    arnygaafsf ladsggWQRW FFAGIAIGIS VILAVMMYrs katqklnnIA
    YALIIGGALG NLFDRLWHGF Vvdmidfyvg dwhfaTFNLA DTAICVGAAL
    IVLEGFLPsr akkq
    MW 18156 Da
    164 aa

     
  • Leader peptidase PppA (pppA)
     

    Peptidase family A24 InterPro
    Function Leader peptidase, removes signal peptides from lipoproteins
    Structure transmemebrane domains: 7 (potential)
     
    TMHMM gives 15-37 102-120 124-141 148-167 177-196 209-231 246-268
    SP gives 0
     
    mlfdvfqqyp tampVLATVG GLIIGSFLNV VIWrypimlr qqmaefhgem
    ssaqskisla lprshcphcq qtirirdnip lfswlmlkgr crdcqakisk
    rYPLVELLTA LAFLLASLVW pesgWGLAVM ILSAWLIAAS VIdldhqWLP
    DVFTQGVLWT GLIAAWAqqs pltlqdAVTG VLVGFITFYS LRWIAGIVLr
    200
    kealgmgdVL LFAALGGWVG ALSLPNVALI Asccgliyav itkrgstTLP
    FGPCLSLGGI ATLYLQALF
    MW 29466 Da
    269 aa

     
  • Possible Ser protease SohB (sohB)
     

    Peptidase family s49 InterPro
    Function Suppressor of degP(htrA) null mutation
    Structure transmembrane domains: 1 (potential), TMHMM has 2
     
    TMHMM gives 10-32 190-207
    SP gives 9-29
     
    mellseyglF LAKIVTVVLA IAAIAAIIVN VAqrnkrqrg elrvnnlseq
    ykemkeelaa almdshqqkq whkaqkkkhk qeakaakaka klgevatdsk
    prvwvldfkg smdahevnsl reeitavlaa fkpqdqvvlr lespggmvhg
    yglaasqlqr lrdknipltv tvdkvaasgg ymmacvadki vsapfaivgs
    200
    igvvaqmpnf nrflkskdid ielhtagqyk rtltllgent eegrekfree
    lnethqlfkd fvkrmrpsld ieqvatgehw ygqqavekgl vdeintsdev
    ilslmegrev vnvrymqrkr lidrftgsaa esadrlllrw wqrgqkplm
    MW 39366 Da
    349 aa

     
  • Signal peptide peptidase/Protease IV (sppA)
     

    Peptidase family s49 InterPro
    Function Degrades cleaved signal peptides
    Structure transmembrane domains: 3 (potential), TMHMM has 1
     
    TMHMM gives 21-43
    SP gives 29-45 398-414 421-441
     
    mrtlwrfiag ffkwtwrlln FVREMVLNLF FIFLVLVGVG IWMqvsggds
    ketasrgall ldisgvivdk pdssqrfskl srqllgassd rlqenslfdi
    vntirqakdd rnitgivmdl knfaggdqps mqyigkalke frdsgkpvya
    vgenysqgqy ylasfankiw lspqgvvdlh gfatnglyyk slldklkvst
    200
    hvfrvgtyks avepfirddm spaareadsr wigelwqnyl ntvaanrqip
    aeqvfpgaqg llegltktgg dtakyalenk lvdalassae iekaltkefg
    wsktdknyra isyydyalkt padtgdsigv vfangaimdg eetqgnvggd
    ttaaqirdar ldpkvkaivl rvnspggtvt aseviraela aaraagkpVV
    400
    VSMGGMAASG GYWIstpanY IVANPSTLTG SIGIFGVITT Vensldsigv
    htdgvstspl advsitralp peaqlmmqls iengykrfit lvadarhstp
    eqidkiaqgh vwtgqdakan glvdslgdfd davakaaela kvkqwhleyy
    vdeptffdkv mdnmsgsvra mlpdafqaml paplasvast vksesdklaa
    600
    fndpqnryaf cltcanmr
    MW 67233 Da
    618 aa

     
  • rseP (YaeL)
     

    Peptidase family M50B
    Function RIP protease, degrades the antisigma factor RseA
    Structure transmembrane domains: 4 (potential), SP has 3
     
    TMHMM gives 2-21 98-120 376-398 426-445
    SP gives 107-127 376-396 430-450
     
    mLSFLWDLAS FIVALGVLIT Vhefghfwva rrcgvrverf sigfgkalwr
    rtdklgteyv ialiplggyv kmlderaepv vpelrhhafn nksvgqrAAI
    IAAGPVANFI FAIFAYWLVF iigvpgvrpv vgeiaansia aeaqiapgte
    lkavdgietp dwdavrlqlv dkigdestti tvapfgsdqr rdvkldlrhw
    200
    afepdkedpv sslgirprgp qiepvlenvq pnsaaskagl qagdrivkvd
    gqpltqwvtf vmlvrdnpgk slaleierqg splsltlipe skpgngkaig
    fvgiepkvip lpdeykvvrq ygpfnaivea tdktwqlmkl tvsmlgklit
    gdvklnnlsg pisiakgagm taelgVVYYL PFLALISVNL GIINLFPLpv
    400
    ldgghllfla iekikggpvs ervqdFCYRI GSILLVLLMG LALFNdfsrl
    MW 49071 Da
    450 aa

     
  • YfbL (yfbL)
     

    Peptidase family M28A
    Function  
    Structure transmembrane domains: 1 (potential)
     
    TMHMM gives 5-27
    SP gives 4-24
     
    mkkIIFAFII LFVFLLPMII FYQPWVnalp stprhaspeq lektvryltq
    tvhprsadni dnlnrsaeyi kevfvssgar vtsqdvpitg gpyknivady
    gpadgpliii gahydsassy endqltytpg addnasgvag llelarllhq
    qvpktgvqlv ayaseeppff rsdemgsavh aaslerpvkl mialemigyy
    200
    dsapgsqnyp ypamswlypd rgdfiavvgr iqdinavrqv kaallssqdl
    svysmntpgf ipgidfsdhl nywqhdipai mitdtafyrn kqyhlpgdta
    drlnyqkmaq vvdgvitlly nsk
    MW 35931 Da
    323 aa

     

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Periplasm

 

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Outer membrane

 

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