Funding
Andor/Nikon Spinning Disk confocal microscope was funded by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) – Project-ID 181168673.
Microscope and Accessories
- inverted Nikon Ti-E
- Perfect Focus System PFS
- motorized xy-stage
- Prior Scientific NanoScanZ Piezo stage
- external fluorescence lamp Nikon Intensilight
- Halogen lamp for transmitted light
- EMBLEM microscope incubator box with control device (temperature & CO2 control for live-cell imaging)
Lasers
Andor 4 line ILE multimode
- Solid state 405nm smart diode laser (100 mW)
- Solid state 488nm smart diode laser (50 mW)
- Solid state 561nm smart diode laser (50 mW)
- Solid state 637nm smart diode laser (140 mW)
Dual Spinning Disk Confocal Scanner
- Yokogawa CSU-X1 Spinning Disk unit, max. 5000 rpm disk spin speed/1000 frames per second
- Andor MicroPoint 4 Photostimulation & Optogenetics Tool
Cameras
- Andor Technology ZL 41 Cell sCmos, 4.1 Megapixel, up to 100 fps, up to 100 nm resolution, up to 82% QE
Objectives
| name |
magnification
|
NA |
immersion |
| CFI Plan Apo 60x/1.2 |
60x |
1.2 |
water |
| CFI Pl Apo VC 20x/0.75 |
20x |
0.75 |
dry |
| CFI Pl Apo 40x/0.95 |
40x |
0.95 |
dry |
| CFI Apo TIRF 60x/1.49 |
60x |
1.49 |
oil |
| CFI Plan Apo Lambda S100x/1.35 |
100x |
1.35 |
silicone oil |
Fluorescence filters & Dichroics
for confocal imaging (emission discrimination and filters):
- CSU Quad Dichroic mirror set for laser wavelength 405 nm, 488 nm, 561 nm, 640 nm
- single band emission filters:
- DAPI: BP 447/60 nm (Semrock BrightLine)
- RFP: BP 617/73 nm (Semrock BrightLine)
- Cy5: BP 685/40 nm (Semrock BrightLine)
- dual band emission filters:
- GFP/RFP: BP 512/23 nm + BP 630/91 nm (Semrock BrightLine)
- GFP/Cy5: BP 538/50 nm + BP 685/45 nm (Semrock BrightLine)
for widefield imaging (filter sets):
- DAPI: EX 340-380, DM 400, BA 435-485
- FITC: EX 465-495, DM 505, BA 515-555
- TRITC: EX 540/25, DM 565, BA 605/55
