Microscope and Accessories

• inverted Zeiss Axio Observer 7
• Colibri 7 multi-color LED illumination for fluorescence: 385/430/475/511/555/590/630 nm
• microLED for transmitted light
• mechanical stage 130x85R
• LD condenser 0.55 H, Ph1, Ph2, Ph3, DIC, DIC; 6 positions, mot.

Cameras

Zeiss AxioCam 506 mono CCD camera, 2752 x 2208 imaging array, 4.54 µm x 4.54 µm pixels, 14-bit digitization

Objectives

Fluorescence filter sets

• Set 01 UV: Ex BP 365/12, FT 395, Em LP 397 (e.g. DAPI, Hoechst)
• Set 47: Ex BP 436/20, FT 455, Em BP 480/40 (e.g. ECFP)
• Set 38 HE: Ex BP 470/40, FT 495, Em BP 525/50 (e.g. EGFP, Alexa Fluor 488, FITC)
• Set 46: Ex BP 500/20, FT 515, Em BP 535/30 (e.g. EYFP, Alexa Fluor 488, Citrine)
• Set 15: Ex BP 546/12, FT 580, Em LP 590 (e.g. TAMRA, Rhodamine, Alexa Fluor 555)
• Set 50: Ex BP 640/30, FT 660, Em BP 690/50 (e.g. Cy5, Alexa Fluor 647, Atto 647)

ZEN 2.3 (Zeiss) with software modules Measurement, Multi Channel, Panorama, Manual Extended Focus, Z-Stack, Extended Focus and Image Analysis