Microscope and Accessories

• inverted Zeiss AxioObserver Z1
• motorized xy-stage
• external fluorescence lamp, mercury burner (100 W)
• Halogen lamp for transmitted light
• Micromanipulation unit: LN manipulator block xyz, motorized for microinjection; AIS 2 FemtoJet
• EMBLEM microscope incubator box with control device (temperature & CO2 control for live-cell imaging)

Cameras

• The Imaging Source DMK31BF03 CCD camera, 1024 x 768 imaging array, 4.65 x 4.65-µm pixels, 8-bit digitization
• Zeiss AxioCam MRm CCD camera, 1388 x 1040 imaging array, 6.45 x 6.45-µm pixels, 12-bit digitization

Objectives

Fluorescence filter sets

• Set 01 UV: Ex BP 365/12, FT 395, Em LP 397 (e.g DAPI, Hoechst)
• Set 10: Ex BP 450-490, FT 510, Em BP 515-565 (e.g. EGFP, Alexa Fluor 488, FITC)
• Set 15: Ex BP 546/12, FT 580, Em LP 590 (e.g. TAMRA, Rhodamine, Alexa Fluor 555)
• Set 26: Ex BP 575-625, FT 645, Em BP 660-710 (e.g mPlum, Nile Red)

Zeiss ZEN 2.0