ICCE Equipment - Zeiss AxioObserver Z.1 microinjection
Widefield Systems Zeiss AxioObserver Z.1 microinjection
Room: S05 V04 E65
Main Applications
- widefield system for epi-fluorescence and transmitted light applications (brightfield)
- multi-dimensional live cell + fixed specimen imaging
- computer-assisted microinjection
Technical Specifications
Microscope and Accessories
- inverted Zeiss AxioObserver Z1
- motorized xy-stage
- external fluorescence lamp, mercury burner (100 W)
- Halogen lamp for transmitted light
- Micromanipulation unit: LN manipulator block xyz, motorized for microinjection; AIS 2 FemtoJet
- EMBLEM microscope incubator box with control device (temperature & CO2 control for live-cell imaging)
Cameras
- The Imaging Source DMK31BF03 CCD camera, 1024 x 768 imaging array, 4.65 x 4.65-µm pixels, 8-bit digitization
- Zeiss AxioCam MRm CCD camera, 1388 x 1040 imaging array, 6.45 x 6.45-µm pixels, 12-bit digitization
Objectives
| name |
magnification |
NA | immersion |
| A-Plan 5x/0.12 | 5x | 0.2 | dry |
| LD Plan-Neofluar 20x/0.4 Corr Ph2 M27 | 20x | 0.4 | dry |
| LD Plan-Neofluar 40x/0.6 Corr Ph2 M27 | 40x | 0.6 | dry |
| Plan-Apochromat 63x/1.40 Oil | 63x | 1.4 | oil |
Fluorescence filter sets
- Set 01 UV: Ex BP 365/12, FT 395, Em LP 397 (e.g DAPI, Hoechst)
- Set 10: Ex BP 450-490, FT 510, Em BP 515-565 (e.g. EGFP, Alexa Fluor 488, FITC)
- Set 15: Ex BP 546/12, FT 580, Em LP 590 (e.g. TAMRA, Rhodamine, Alexa Fluor 555)
- Set 26: Ex BP 575-625, FT 645, Em BP 660-710 (e.g mPlum, Nile Red)
Software
Zeiss ZEN 2.0