Microscope and Accessories

• inverted Nikon Ti2-E
• Perfect Focus System PFS4 (hardware autofocus system)
• motorized xy-stage
• Nikon Ti LAPP system with modules:
  • epi-fluorescence
  • H-TIRF for fully automated TIRF adjustments
  • FRAP for photobleaching and photoactivation/conversion experiments
• okolab bold line cage incubator with control device (temperature & CO2 control for live-cell imaging)

Cameras and TuCam

• 2 x: Andor Technology iXon Life DU-888 back-illuminated EMCCD camera, 1024 x1024 pixels imaging array, 13 x 13 µm pixel size, 13.3 x 13.3 mm sensor format, 16-bit digitization, 56 fps at full frame
• Andor TuCam Dual Camera Imaging adapter, (GFP/mCherry: Brightline HC 525/50, Brightline HC 609/54, Beamsplitter R561)

Light sources

• Lumencor Sola SE II solid state white light illumination
• LED transmitted light module

Lasers

Omicron Light Hub-4 compact laser beam combiner with AOTF laser modulator and fast fiber switch with two fiber outputs (TIRF & FRAP)

• Diode Laser cw LuxX 405 nm (120 mW)
• Diode Laser cw LuxX 488 nm (250 mW)
• DPSS Laser cw Jive 561 nm (300 mW)
• Diode Laser cw LuxX 638 nm (200 mW)

Fluorescence filters & dichroics

Filter cubes for LED epifluorescence

Quadband dichroic and emission filters for TIRF

Objectives

Nikon Imaging software NIS Elements AR (Advanced Research)