Funding
DeltaVision Elite widefield deconvolution microscope was funded by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) – Project-ID 361068552.
Microscope and Accessories
- Inverted Olympus IX-71 microscope stand
- UltimateFocus Modul (hardware autofocus system)
- Automated high precision Flexure stage (long travel, 25x50 mm), “3D multisite visiting”
- Environmental chamber with temperature & CO2 control for live cell imaging
Camera
- pco.edge 5.5 sCMOS camera, 2560 x 2160 pixels, 6.5 x 6.5 µm pixel size, 16.6 x 14 mm sensor format, 16-bit digitization, 200 fps at 1024 x 1024
Light sources
- DV Light SSI 7 color ultra-fast solid state illumination
excitation filters
| Fluorochrome |
excitation bandpass [nm] |
| DAPI |
390/18 |
| CFP |
438/24 |
| GFP/TRITC |
475/28 |
| YFP |
513/17 |
| TRITC |
542/27 |
| mCherry/Alexa594 |
575/25 |
| Cy5 |
632/22 |
- LED transmitted light module
Emission filters & Polychroic beamsplitters
Single band emission filters
| Fluorochrome |
emission bandpass [nm] |
| DAPI |
435/48 (use with Polychroic #1 or #2) |
| CFP |
475/24 (use with Polychroic #3) |
| GFP/TRITC |
525/48 (use with Polychroic #1 or #2) |
| YFP |
548/22 (use with Polychroic #3) |
| TRITC |
597/45 (use with Polychroic # 1) |
| mCherry/Alexa594 |
625/45 (use with Polychroic #2 or #3) |
| Cy5 |
679/37 (use with Polychroic #1 or #2) |
Polychroic beamsplitters
| Position |
Fluorochromes |
| Polychroic #1 |
DAPI/FITC/TRITC/Cy5 |
| Polychroic #2 |
DAPI/FITC/mCherry/Alexa594/Cy5 |
| Polychroic #3 |
CFP/YFP/mCherry |
Objectives
| name |
magnification
|
NA |
immersion |
| Super-Plan Apo 20x/0.75 DIC |
20x |
0.75 |
dry |
| Plan Apo 60x/1.42 oil DIC |
60x |
1.42 |
oil |
| Super-Plan Apo 100x/1.4 oil DIC |
100x |
1.4 |
oil |
SoftWoRx 6 and Resolve 3D (integrated in SoftWoRx) with software modules for Deconvolution (image restoration), Cell tracking, Point visiting, Optic axial integration (OAI) and 3D visualization
