Aria III
Light microscopyFlow cytometry and cell sorting
Technical Details:
The BD FACS Aria III is a high speed cell sorter.
For more detailed technical specifications have a look here.
Type:
5 Laser with aerosole management system.
Lasers:
405nm, or 365 Diode laser, 488nm solid state, 561nm solid state and 633nm HENE.
Detectors:
Forward scatter.
Laser 1: Octagon with 3 fluorescence detectors
Laser 2: Trigon with 3 fluorescence detectors and side scatter
Laser 3: Trigon with 3 fluorescence detectors
Laser 4: Octagon with 5 fluorescence detectors
Software: BD Diva
Filtersettings:
|
Laser |
Position |
LP |
Filter |
Common dyes |
|---|---|---|---|---|
|
405/365nm Octragon |
A |
600 |
610/20 |
QDot 605 |
|
violett laser |
B |
502 |
510/50 |
AmCyan; HorizonV500; Alexa 430 |
|
C |
450/40 |
Dapi; Hoechst; Pacific Blue; AF405 |
||
|
633nm Trigon |
A |
755 |
780/60 |
APC-Cy7 |
|
red laser |
B |
690 |
730/45 |
Alexa700 |
|
C |
660/20 |
TOPRO3; Alexa674; APC |
||
|
488nm Trigon |
A |
655 |
695/40 |
PerCP5.5; PI; PeCy5; PerCP |
|
blue laser |
B |
502 |
530/30 |
FITC; GFP; AF488 |
|
C |
488/10 |
(SSC) |
||
|
561nm Octagon |
A |
735 |
780/60 |
PeCy7 |
|
yellow/green laser |
B |
685 |
710/50 |
PeCy5.5 |
|
C |
630 |
670/14 |
PeCy5 |
|
|
D |
600 |
610/20 |
mCherry; PeTexasRed; PI; Alexa 568 |
|
|
E |
582/15 |
PE; Alexa555; Cy3; RFP |
Sort collection devices:
|
Room temperature |
|
|
2-way |
12x75mm |
|
15ml tubes |
|
|
4-way |
12x75mm |
|
1,5ml tubes |
|
|
Adjustable temperature (5°C-40°C) |
|
|
2-way |
15ml tubes |
|
4-way |
12x75mm tubes |
|
1,5ml tubes |
|
Please Note: A FACSFusion cell sorter (a ARIA3-like sorter integrated into a laminar flow) is operated at the IFZ. Further details about this sorter can be found at:
https://www.uk-essen.de/zellbiologie/institut/zellsortierer-einrichtung/