Download our operating protocol.

And take a look to the whole mount staining iDISCO-Protocol (vers. 2016)

Technical Details:

LaVision BioTec Ultramicroscope fluorescence light sheet imaging for 3D image reconstruction in large samples . Cleared wholemount specimens are illuminated using a thin light sheet and imaged on a high resolution sCMOS camera orthogonal to illumination. Selective illumination ensures decreased photobleaching. System is equipped with dual lightsheet illumination.

Microscope:
Is build by Milteniy Biotec with three available objectives (1.1x, 4x and 12x) additionally you can choose a Zoom of 0.6x, 1x or 2.5x for each objective.

Light sheet excitation lines:
White Light laser (200mW) 480-2400nm

Camera:
Andor Neo sCMOS camera: scientific low noise sCMOS camera (6.5µmx6.5µm pixel size).

Detection Filters: