Leica MP & FLIM

Light MicroscopyLeica MP & FLIM

Technical Details:

 

 

Microscope:

Leica TCS SP8 fully automated epifluorescence confocal microscope, with AOTF and AOBS, gated HyD detection, multi Photon (MP) and Fluorescence Lifetime Imaging - (FLIM).

On a DM6000 CFS basic frame.

 

Confocal Scanner:

up to 8 laserlines with AOBS and VISIR scanoptic with scanrotation. 12kHZ tandemscanner (confocal pointscanner/true confocal scanning system) with 3 channel spectral detection consisting of 2PMT and 1 HyD. Detection region 400-800nm.

 

Nonresonant mode:

Speed  1-1800 Hz

X2Y Scanner Design for maximum scanfield

Max. 7 pic. / sec. 512 x 512

Max  84 pic. / sec. 512 x 16

Max. resolution 8192 x 8192 or 64 Mpixel

 

Resonant mode:

Linefrequency: 24000 Hz (bidirectional)

Frame rate 512x512 pixel (full screen) up to 40 pic. / sec.

Frame rate bei 512x16 pixel bis zu 428 pic. / sec.

Scan format max.: 832 x 832 pixel

Scan format min.: 16 x 16 pixel

Laser excitation lines:

confocal:

  • Argon Ion (65 mW): 458nm,476nm, 488nm, 496nm, 514nm. 
  • HeNe (10 mW) 633nm
  • DPSS (20mW) 561nm

Multi photon:

  • Titan:Saphir laser:

  Chameleon Vision II tunable from 680-1040nm (in simultaneous mode 760-860nm)

  with >2,8 W power (at 800 nm)

  Pulse width ~140 fs

  • Compact OPO tunable from 1030-1280nm with >500 mW power (at 1100 nm)

  Pulse width ~200 fs

 

2 non descanned reflectet light detection filter sets with 2 HyD and 2 PMT:

Set 1 DAPI/FITC/TRITC/AF633

 
  Filter Beamsplitter
Cube 1 DAPI / FITC PMT Detektor BP1 460/50 BS RSP495
  BP2 525/50  
Cube 2 TRITC / AF633 HyD BP1 585/40 BS RSP 620
  BP2 650/50  
Beamsplitter   BS RSP 560

 

Set2 SHG 440 CFP / YFP / DSRED

 
  Filter Beamsplitter
Cube 1 SHG 440 / CFP PMT Detektor BP1 440/20 BS RSP 455
  BP2 483/32  
Cube 2 YFP / DSRED HyD BP1 525/30 BS RSP 560
  BP2 585/40  
Beamsplitter   BS RSP 505

 

 

Fluoreszenzlebensdauer Mikroskopie SP FLIM:
TCSPC Modul PicoHarp 300, Analyse Software "SymphoTime" SPT2.