Download our operating protocol.

And take a look to the whole mount staining iDISCO-Protocol (vers. 2016)

Technical Details:

LaVision BioTec Ultramicroscope fluorescence light sheet imaging for 3D image reconstruction in large samples . Cleared wholemount specimens are illuminated using a thin light sheet and imaged on a high resolution sCMOS camera orthogonal to illumination. Selective illumination ensures decreased photobleaching. System is equipped with dual lightsheet illumination.

Microscope:
Based around an Olympus MVX10 zoom microscope body (variable zoom 0.63 - 6.3 X)

Light sheet excitation lines:
OPSL (50mW), 488nm.
OPSL (50mW), 561nm.
Diode laser (50mW), 647nm
Diode (50mW) 785nm

Camera:
Andor Neo sCMOS camera: scientific low noise CMOS camera.

Detection Channels: