EMU Instruments for Scanning Electron Microscopy

MFZ Building 1st floor EMU Room 1.039 Scanning Electron Microscope (SEM) ZEISS Crossbeam 540 FIB

Cryo-SEM with capella column with Ga+++ FIB, in-lens, SE2- and energy selective detectors, STEM, GIS, resolution 0.7 nm at 30KV with Atlas software package
for
- standard scanning electron microscopy
- focused ion beam investigations --> 3D reconstructions
- cryo scanning electron microscopy

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MFZ Building 1st floor EMU Room 1.039 Cryo Scanning Electron Microscopy Preparation System Quorum PP3010T

Preparation table + preparation chamber (Quorum PP3010T) which is directly flunshed onto the SEM
for
- cryo scanning electron microscopy
- freeze etching and fracturing scanning electron microscopy

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MFZ Building 1st floor EMU Room 1.035 Critical Point Dryer Leica EM CPD 300

With this device scanning electron microscopic samples are critical-point-dried which is necessary before sputtering

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MFZ Building 1st floor EMU Room 1.037 Sputter-Coater Leica ACE 600

With this device the surfaces of specimens are sputter coated with metals (platinum, palladium, gold or chrome) for better visualisation.

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MFZ Building 1st floor EMU Room 1.039 Sputter-Coater Quorum Q 150TES

For some samples it is reasonable to perform carbon sputter coating before investigation on the scanning electron microscope. This device exclusively is used for that purpose.

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MFZ Building 1st floor EMU Room 1.037 Laboratory microwave Bio Wave Pro Plus

Lab microwave with magnetic stirrer and vaccum system for
- quick embedding of electron microscopic samples
- treatment of tissue before paraffin- or immune fluorescence embedding
- decalcification (e.g., of bones)

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MFZ Building 2nd floor EMU Room 2.013 Workstations for image processing & reconstructions

High-end workstations for professional image processing & segmentation
for
- working with + segmentation of large image stacks
- three-dimensional visualisation of segmented structures

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