ZMB Member Andrea Musacchio

ZMB Member
Andrea Musacchio

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portrait photo of ZMB member Andrea Musacchio
© MPI/Musacchio

Prof. Dr. Andrea Musacchio

Group

Mechanistic Cell Biology

Max Planck Institute of Molecular Physiology
Otto-Hahn-Staße 11
44227 Dortmund

Research Overview

  • Spindle assembly checkpoint components and functions
  • Spindle assembly checkpoint (SAC) signalling
  • Mitotic checkpoint complex (MCC)
  • Anaphase-promoting complex (APC/C)
  • Structural organization of the kinetochore
  • Analyses of localization dependencies of kinetochore proteins
  • Kinetochore receptors of checkpoint components
  • Kinetochore–microtubule attachment
  • Homeostatic control of mitotic arrest
  • Sensitivity of cancer cells to antitubulin chemotherapeutics

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Selected Publications

    Journal articles

  • Hedtfeld, Marius; Dammers, Alicia; Koerner, Carolin; Musacchio, Andrea
    A validation strategy to assess the role of phase separation as a determinant of macromolecular localization
    In: Molecular Cell Vol. 84 (2024) Nr. 9, pp. 1783 - 1801.e7
    Online Full Text: dx.doi.org/ (Open Access)
  • Conti, Duccio; Verza, Arianna Esposito; Pesenti, Marion E.; Cmentowski, Verena; Vetter, Ingrid R.; Pan, Dongqing; Musacchio, Andrea
    Role of protein kinase PLK1 in the epigenetic maintenance of centromeres
    In: Science Vol. 385 (2024) Nr. 6713, pp. 1091 - 1097
    Online Full Text: dx.doi.org/
  • Polley, Soumitra; Raisch, Tobias; Ghetti, Sabrina; Körner, Marie; Terbeck, Melina; Gräter, Frauke; Raunser, Stefan; Aponte-Santamaría, Camilo; Vetter, Ingrid R.; Musacchio, Andrea
    Structure of the human KMN complex and implications for regulation of its assembly
    In: Nature Structural & Molecular Biology Vol. 31 (2024) Nr. 6, pp. 861 - 873
    Online Full Text: dx.doi.org/ (Open Access)
  • Musacchio, Andrea
    Thirty years of structural changes
    In: Nature Structural & Molecular Biology Vol. 31 (2024) Nr. 1, pp. 4 - 5
    Online Full Text: dx.doi.org/
  • Cmentowski, Verena; Ciossani, Giuseppe; d'Amico, Ennio; Wohlgemuth, Sabine; Owa, Mikito; Dynlacht, Brian; Musacchio, Andrea
    RZZ‐Spindly and CENP‐E form an integrated platform to recruit dynein to the kinetochore corona
    In: The EMBO Journal Vol. 42 (2023) Nr. 24, e114838
    Online Full Text: dx.doi.org/ (Open Access)
  • Polley, Soumitra; Müschenborn, Helen; Terbeck, Melina; De Antoni, Anna; Vetter, Ingrid R; Dogterom, Marileen; Musacchio, Andrea; Volkov, Vladimir A; Huis in 't Veld, Pim J
    Stable kinetochore-microtubule attachment requires loop-dependent Ndc80-Ndc80 binding
    In: The EMBO Journal Vol. 42 (2023) Nr. 13, e112504
    Online Full Text: dx.doi.org/ (Open Access)
  • Musacchio, Andrea
    On the role of phase separation in the biogenesis of membraneless compartments
    In: The EMBO Journal Vol. 41 (2022) Nr. 5, e109952
    Online Full Text: dx.doi.org/ (Open Access)
  • Raisch, Tobias; Ciossani, Giuseppe; d’Amico, Ennio; Cmentowski, Verena; Carmignani, Sara; Maffini, Stefano; Merino, Felipe; Wohlgemuth, Sabine; Vetter, Ingrid R.; Raunser, Stefan; Musacchio, Andrea
    Structure of the RZZ complex and molecular basis of Spindly‐driven corona assembly at human kinetochores
    In: The EMBO Journal Vol. 41 (2022) Nr. 9, e110411
    Online Full Text: dx.doi.org/ Online Full Text (Open Access)
  • Pesenti, Marion E.; Raisch, Tobias; Conti, Duccio; Walstein, Kai; Hoffmann, Ingrid; Vogt, Dorothee; Prumbaum, Daniel; Vetter, Ingrid R.; Raunser, Stefan; Musacchio, Andrea
    Structure of the human inner kinetochore CCAN complex and its significance for human centromere organization
    In: Molecular Cell Vol. 82 (2022) Nr. 11, pp. 2113 - 2131.e8
    Online Full Text: dx.doi.org/ (Open Access)
  • Singh, Priyanka; Pesenti, Marion E.; Maffini, Stefano; Carmignani, Sara; Hedtfeld, Marius; Petrovic, Arsen; Srinivasamani, Anupallavi; Bange, Tanja; Musacchio, Andrea
    BUB1 and CENP-U, Primed by CDK1, Are the Main PLK1 Kinetochore Receptors in Mitosis
    In: Molecular Cell Vol. 81 (2021) Nr. 1, pp. 67 - 87.e9
    Online Full Text: dx.doi.org/ (Open Access)
  • Piano, Valentina; Alex, Amal; Stege, Patricia; Maffini, Stefano; Stoppiello, Gerardo A.; Huis In'T Veld, Pim J.; Vetter, Ingrid R.; Musacchio, Andrea
    CDC20 assists its catalytic incorporation in the mitotic checkpoint complex
    In: Science Vol. 371 (2021) Nr. 6524, pp. 67 - 71
    Online Full Text: dx.doi.org/
  • van den Boom, Johannes; Kück, Anja; Kravic, Bojana; Müschenborn, Helen; Giesing, Maike; Pan, Dongqing; Kaschani, Farnusch; Kaiser, Markus; Musacchio, Andrea; Meyer, Hemmo
    Targeted substrate loop insertion by VCP/p97 during PP1 complex disassembly
    In: Nature Structural & Molecular Biology Vol. 28 (2021) Nr. 12, pp. 964 - 971
    Online Full Text: dx.doi.org/
  • Pesenti, Marion E; Prumbaum, Daniel; Auckland, Philip; Smith, Charlotte M; Faesen, Alex C; Petrovic, Arsen; Erent, Muriel; Maffini, Stefano; Pentakota, Satyakrishna; Weir, John R; Lin, Yu-Chih; Raunser, Stefan; McAinsh, Andrew D; Musacchio, Andrea
    Reconstitution of a 26-Subunit Human Kinetochore Reveals Cooperative Microtubule Binding by CENP-OPQUR and NDC80.
    In: Molecular Cell Vol. 71 (2018) Nr. 6, pp. 923 - 939.e10
    Online Full Text: dx.doi.org/ (Open Access)
  • Weith, Matthias; Seiler, Jonas; van den Boom, Johannes; Kracht, Matthias; Hülsmann, Julia; Primorac, Ivana; Del Pino Garcia, Javier; Kaschani, Farnusch; Kaiser, Markus; Musacchio, Andrea; Bollen, Mathieu; Meyer, Hemmo
    Ubiquitin-Independent Disassembly by a p97 AAA-ATPase Complex Drives PP1 Holoenzyme Formation
    In: Molecular Cell Vol. 72 (2018) Nr. 4, pp. 766 - 777
    Online Full Text: dx.doi.org/ (Open Access)
  • Faesen, Alex C.; Thanasoula, Maria; Maffini, Stefano; Breit, Claudia; Müller, Franziska; Van Gerwen, Suzan; Bange, Tanja; Musacchio, Andrea
    Basis of catalytic assembly of the mitotic checkpoint complex
    In: Nature Vol. 542 (2017) Nr. 7642, pp. 498 - 502
    Online Full Text: dx.doi.org/ Online Full Text (Open Access)
  • Weir, John R.; Faesen, Alex C.; Klare, Kerstin; Petrovic, Arsen; Basilico, Federica; Fischböck, Josef; Pentakota, Satyakrishna; Keller, Jenny; Pesenti, Marion E.; Pan, Dongqing; Vogt, Doro; Wohlgemuth, Sabine; Herzog, Franz; Musacchio, Andrea
    Insights from biochemical reconstitution into the architecture of human kinetochores
    In: Nature Vol. 537 (2016) Nr. 7619, pp. 249 - 253
    Online Full Text: dx.doi.org/
  • Petrovic, Arsen; Keller, Jenny; Liu, Yahui; Overlack, Katharina; John, Juliane; Dimitrova, Yoana N.; Jenni, Simon; van Gerwen, Suzan; Stege, Patricia; Wohlgemuth, Sabine; Rombaut, Pascaline; Herzog, Franz; Harrison, Stephen C.; Vetter, Ingrid R.; Musacchio, Andrea
    Structure of the MIS12 Complex and Molecular Basis of Its Interaction with CENP-C at Human Kinetochores
    In: Cell Vol. 167 (2016) Nr. 4, pp. 1028 - 1040.e15
    Online Full Text: dx.doi.org/ (Open Access)
  • Faesen, Alex C.; Musacchio, Andrea
    The (phospho) needle in the (MELT) haystack
    In: Molecular Cell Vol. 57 (2015) Nr. 5, pp. 765 - 766
    Online Full Text: dx.doi.org/ (Open Access)
  • Eiteneuer, Annika; Seiler, Jonas; Weith, Matthias; Beullens, Monique; Lesage, Bart; Krenn, Veronica; Musacchio, Andrea; Bollen, Mathieu; Meyer, Hemmo
    Inhibitor-3 ensures bipolar mitotic spindle attachment by limiting association of SDS22 with kinetochore-bound protein phosphatase-1
    In: The EMBO Journal Vol. 33 (2014) Nr. 22, pp. 2704 - 2720
    Online Full Text: dx.doi.org/ Online Full Text (Open Access)
  • Petrovic, Arsen; Mosalaganti, Shyamal; Keller, Jenny; Mattiuzzo, Marta; Overlack, Katharina; Krenn, Veronica; De Antoni, Anna; Wohlgemuth, Sabine; Cecatiello, Valentina; Pasqualato, Sebastiano; Raunser, Stefan; Musacchio, Andrea
    Modular Assembly of RWD Domains on the Mis12 Complex Underlies Outer Kinetochore Organization
    In: Molecular Cell Vol. 53 (2014) Nr. 4, pp. 591 - 605
    Online Full Text: dx.doi.org/ (Open Access)
  • Musacchio, Andrea; Ciliberto, Andrea
    The spindle-assembly checkpoint and the beauty of self-destruction : Erratum
    In: Nature Structural & Molecular Biology Vol. 20 (2013) Nr. 2, pp. 244
    Online Full Text: dx.doi.org/
  • Musacchio, Andrea; Ciliberto, Andrea
    The spindle-assembly checkpoint and the beauty of self-destruction
    In: Nature Structural & Molecular Biology Vol. 19 (2012) Nr. 11, pp. 1059 - 1061
    Online Full Text: dx.doi.org/
  • Santaguida, Stefano; Vernieri, Claudio; Villa, Fabrizio; Ciliberto, Andrea; Musacchio, Andrea
    Evidence that Aurora B is implicated in spindle checkpoint signalling independently of error correction
    In: The EMBO Journal Vol. 30 (2011) Nr. 8, pp. 1508 - 1519
    Online Full Text: dx.doi.org/
  • Varetti, Gianluca; Guida, Claudia; Santaguida, Stefano; Chiroli, Elena; Musacchio, Andrea
    Homeostatic control of mitotic arrest
    In: Molecular Cell Vol. 44 (2011) Nr. 5, pp. 710 - 720
    Online Full Text: dx.doi.org/
  • Musacchio, Andrea
    Travel notes from the equatorial circle
    In: Cell Vol. 146 (2011) Nr. 4, pp. 499 - 501
    Online Full Text: dx.doi.org/
  • Santaguida, Stefano; Musacchio, Andrea
    The life and miracles of kinetochores
    In: The EMBO Journal Vol. 28 (2009) Nr. 17, pp. 2511 - 2531
    Online Full Text: dx.doi.org/
  • Ciferri, Claudio; Pasqualato, Sebastiano; Screpanti, Emanuela; Varetti, Gianluca; Santaguida, Stefano; Dos Reis, Gabriel; Maiolica, Alessio; Polka, Jessica; De Luca, Jennifer G.; De Wulf, Peter; Salek, Mogjiborahman; Rappsilber, Juri; Moores, Carolyn A.; Salmon, Edward D.; Musacchio, Andrea
    Implications for kinetochore-microtubule attachment from the structure of an engineered Ndc80 complex
    In: Cell Vol. 133 (2008) Nr. 3, pp. 427 - 439
    Online Full Text: dx.doi.org/
  • Mapelli, Marina; Massimiliano, Lucia; Santaguida, Stefano; Musacchio, Andrea
    The Mad2 conformational dimer : structure and implications for the spindle assembly checkpoint
    In: Cell Vol. 131 (2007) Nr. 4, pp. 730 - 743
    Online Full Text: dx.doi.org/
  • Penengo, Lorenza; Mapelli, Marina; Murachelli, Andrea G.; Confalonieri, Stefano; Magri, Laura; Musacchio, Andrea; Di Fiore, Pier P.; Polo, Simona; Schneider, Thomas R.
    Crystal structure of the ubiquitin binding domains of rabex-5 reveals two modes of interaction with ubiquitin
    In: Cell Vol. 124 (2006) Nr. 6, pp. 1183 - 1195
    Online Full Text: dx.doi.org/
  • Mapelli, Marina; Filipp, Fabian V.; Rancati, Giulia; Massimiliano, Lucia; Nezi, Luigi; Stier, Gunter; Hagan, Robert S.; Confalonieri, Stefano; Piatti, Simonetta; Sattler, Michael; Musacchio, Andrea
    Determinants of conformational dimerization of Mad2 and its inhibition by p31comet
    In: The EMBO Journal Vol. 25 (2006) Nr. 6, pp. 1273 - 1284
    Online Full Text: dx.doi.org/
  • DeLuca, Jennifer G.; Gall, Walter E.; Ciferri, Claudio; Cimini, Daniela; Musacchio, Andrea; Salmon, Edward D.
    Kinetochore microtubule dynamics and attachment stability are regulated by Hec1
    In: Cell Vol. 127 (2006) Nr. 5, pp. 969 - 982
    Online Full Text: dx.doi.org/
  • Sironi, Lucia; Mapelli, Marina; Knapp, Stefan; De Antoni, Anna; Jeang, Kuan‐Teh; Musacchio, Andrea
    Crystal structure of the tetrameric Mad1-Mad2 core complex : implications of 'safety belt' binding mechanism for the spindle checkpoint
    In: The EMBO Journal Vol. 21 (2002) Nr. 10, pp. 2496 - 2506
    Online Full Text: dx.doi.org/
  • Fraschini, Roberta; Beretta, Alessia; Sironi, Lucia; Musacchio, Andrea; Lucchini, Giovanna; Piatti, Simonetta
    Bub3 interaction with Mad2, Mad3 and Cdc20 is mediated by WD40 repeats and does not require intact kinetochores
    In: The EMBO Journal Vol. 20 (2001) Nr. 23, pp. 6648 - 6659
    Online Full Text: dx.doi.org/
  • Sironi, Lucia; Melixetian, Marina; Faretta, Mario; Prosperini, Elena; Helin, Kristian; Musacchio, Andrea
    Mad2 binding to Mad1 and Cdc20, rather than oligomerization, is required for spindle checkpoint
    In: The EMBO Journal Vol. 20 (2001) Nr. 22, pp. 6371 - 6382
    Online Full Text: dx.doi.org/
  • Kang, Hyun; Freund, Christian; Duke‐Cohan, Jonathan S.; Musacchio, Andrea; Wagner, Gerhard; Rudd, Christopher E.
    SH3 domain recognition of a proline-independent tyrosine-based RKxxYxxY motif in immune cell adaptor SKAP55
    In: The EMBO Journal Vol. 19 (2000) Nr. 12, pp. 2889 - 2899
    Online Full Text: dx.doi.org/
  • Mongioví, Adriana M.; Romano, Pascale R.; Panni, Simona; Mendoza, Manuel; Wong, William T.; Musacchio, Andrea; Cesareni, Gianni; Di Fiore, Pier P.
    A novel peptide-SH3 interaction
    In: The EMBO Journal Vol. 18 (1999) Nr. 19, pp. 5300 - 5309
    Online Full Text: dx.doi.org/
  • Ter Haar, Ernst; Musacchio, Andrea; Harrison, Stephen C.; Kirchhausen, Tomas
    Atomic structure of clathrin : a β propeller terminal domain joins an α zigzag linker
    In: Cell Vol. 95 (1998) Nr. 4, pp. 563 - 573
    Online Full Text: dx.doi.org/
  • Noble, Martin E. M.; Musacchio, Andrea; Saraste, Matti; Courtneidge, Sara A.; Wierenga, Rik K.
    Crystal structure of the SH3 domain in human Fyn : comparison of the three-dimensional structures of SH3 domains in tyrosine kinases and spectrin
    In: The EMBO Journal Vol. 12 (1993) Nr. 7, pp. 2617 - 2624
    Online Full Text
  • Musacchio, Andrea; Noble, Martin; Pauptit, Richard; Wierenga, Rik; Saraste, Matti
    Crystal structure of a Src-homology 3 (SH3) domain
    In: Nature Vol. 359 (1992) Nr. 6398, pp. 851 - 855
    Online Full Text: dx.doi.org/
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