ZMB Member Perihan Nalbant

ZMB Member
Perihan Nalbant

Next ZMB-Member
portrait photo of ZMB member Perihan Nalbant
© UDE/Nalbant

Prof. Dr. Perihan Nalbant

Group

Molecular Cell Biology

Faculty of Biology
University of Duisburg-Essen
Universitätsstr. 2
45141 Essen

Research Overview

A wide range of molecules has been shown to be important for cellular migration. Unfortunately, in cancer, many regulatory processes are aberrant and lead to highly invasive cell behavior and metastasis. In particular, the proteins of the Rho GTPase family, RhoA, Cdc42 and Rac, are most prominently known to be crucial for cell migration and other processes related to cancer invasion. The focus of our research is to understand how spatial and temporal regulation of Rho GTPase activity is organized during cellular migration and how such mechanisms are altered in cancer.
To achieve this, we are using fluorescence biosensor imaging to visualize localized GTPase activity in living cells. The combination of these tools with other contemporary cell biological methods such as siRNA technology is a powerful means to dissect how the complex network of upstream regulators affects localized Rho protein signaling and how this is translated into normal as well as aberrant cell migration.
Read more

Selected Publications

    Journal articles

  • Patwardhan, Rutuja; Nanda, Suchet; Wagner, Jessica; Stockter, Tom; Dehmelt, Leif; Nalbant, Perihan;
    Li, Rong (Eds.)
    Cdc42 activity in the trailing edge is required for persistent directional migration of keratinocytes
    In: Molecular Biology of the Cell Vol. 35 (2023) Nr. 1, br1
    Online Full Text: dx.doi.org/
  • Nalbant, Perihan; Wagner, Jessica; Dehmelt, Leif
    Direct investigation of cell contraction signal networks by light-based perturbation methods
    In: Pflügers Archiv: European Journal of Physiology Vol. 475 (2023) Nr. 12, pp. 1439 - 1452
    Online Full Text: dx.doi.org/
  • Nanda, Suchet; Calderon, Abram; Sachan, Arya; Duong, Thanh-Thuy; Koch, Johannes; Xin, Xiaoyi; Solouk-Stahlberg, Djamschid; Wu, Yao-Wen; Nalbant, Perihan; Dehmelt, Leif
    Rho GTPase activity crosstalk mediated by Arhgef11 and Arhgef12 coordinates cell protrusion-retraction cycles
    In: Nature Communications Vol. 14 (2023) Nr. 1, 8356
    Online Full Text: dx.doi.org/ (Open Access)
  • Kowalczyk, Manuela; Kamps, Dominic; Wu, Yaowen; Dehmelt, Leif; Nalbant, Perihan
    Monitoring the Response of Multiple Signal Network Components to Acute Chemo-Optogenetic Perturbations in Living Cells
    In: ChemBioChem Vol. 23 (2022) Nr. 4, e202100582
    Online Full Text: dx.doi.org/ (Open Access)
  • Hensel, Astrid; Stahl, Paul; Moews, Lisa; König, Lena; Patwardhan, Rutuja; Höing, Alexander; Schulze, Nina; Nalbant, Perihan; Stauber, Roland H.; Knauer, Shirley
    The Taspase1/Myosin1f-axis regulates filopodia dynamics
    In: iScience Vol. 25 (2022) Nr. 6, 104355
    Online Full Text: dx.doi.org/ Online Full Text (Open Access)
  • Dorsch, Madeleine; Kowalczyk, Manuela; Planque, Mélanie; Heilmann, Geronimo; Urban, Sebastian; Dujardin, Philip; Forster, Jan; Ueffing, Kristina; Nothdurft, Silke; Oeck, Sebastian; Paul, Annika; Liffers, Sven T.; Kaschani, Farnusch; Kaiser, Markus; Schramm, Alexander; Siveke, Jens; Winslow, Monte M.; Fendt, Sarah-Maria; Nalbant, Perihan; Grüner, Barbara M.
    Statins affect cancer cell plasticity with distinct consequences for tumor progression and metastasis
    In: Cell Reports Vol. 37 (2021) Nr. 8, 110056
    Online Full Text: dx.doi.org/ Online Full Text (Open Access)
  • Kamps, Dominic; Koch, Johannes; Juma, Victor O.; Campillo-Funollet, Eduard; Gräßl, Melanie; Banerjee, Soumya; Mazel, Tomáš; Chen, Xi; Wu, Yao-Wen; Portet, Stephanie; Madzvamuse, Anotida; Nalbant, Perihan; Dehmelt, Leif
    Optogenetic Tuning Reveals Rho Amplification-Dependent Dynamics of a Cell Contraction Signal Network
    In: Cell Reports Vol. 33 (2020) Nr. 9, pp. 108467
    Online Full Text: dx.doi.org/ (Open Access)
  • Nalbant, Perihan; Dehmelt, Leif
    Exploratory cell dynamics : A sense of touch for cells?
    In: Biological Chemistry Vol. 399 (2018) Nr. 8, pp. 809 - 819
    Online Full Text: dx.doi.org/ (Open Access)
  • Gräßl, Melanie; Koch, Johannes; Calderon, Abram; Kamps, Dominic; Banerjee, Soumya; Mazel, Tomáš; Schulze, Nina; Jungkurth, Jana; Patwardhan, Rutuja; Solouk, Djamschid; Hampe, Nico; Hoffmann, Bernd; Dehmelt, Leif; Nalbant, Perihan
    An excitable Rho GTPase signaling network generates dynamic subcellular contraction patterns
    In: The Journal of Cell Biology (JCB) Vol. 216 (2017) Nr. 12, pp. 4271 - 4285
    Online Full Text: dx.doi.org/ (Open Access)
  • Jeruschke, Stefanie; Jeruschke, Kay; DiStasio, Andrew; Karaterzi, Sinem; Büscher (A.), Anja; Nalbant, Perihan; Klein-Hitpaß, Ludger; Hoyer, Peter Friedrich; Weiss, Jürgen; Stottmann, Rolf W; Weber, Stefanie
    Everolimus Stabilizes Podocyte Microtubules via Enhancing TUBB2B and DCDC2 Expression
    In: PLoS ONE Vol. 10 (2015) Nr. 9, pp. e0137043
    Online Full Text: dx.doi.org/ (Open Access)
  • Jeruschke, Stefanie; Rekasi, Heike; Karaterzi, Sinem; Buescher, AnjaKarin; Klein-Hitpaß, Ludger; Jeruschke, Kay; Weiss, Juergen; Saleem, MoinAhson; Hoyer, Peter Friedrich; Nalbant, Perihan; Weber, Stefanie
    Everolimus Prevents Podocyte Injury Via Stabilizing Tubb2b And Dcdc2 Expression
    In: Pediatric Nephrology Vol. 29 (2014) Nr. 9, pp. 1674 - 1674
    Online Full Text: dx.doi.org/
  • Schulze, Nina; Gräßl, Melanie; Soares, Alexandra Blancke; Geyer, Matthias; Dehmelt, Leif; Nalbant, Perihan
    FHOD1 regulates stress fiber organization by controlling the dynamics of transverse arcs and dorsal fibers
    In: Journal of Cell Science Vol. 127 (2014) Nr. 7, pp. 1379 - 1393
    Online Full Text: dx.doi.org/ (Open Access)
  • Jeruschke, S.; Büscher (A.), Anja; Rekasi, H.; Karaterzi, S.; Saleem, M. A.; Oh, J.; Hoyer, Peter Friedrich; Nalbant, Perihan; Weber, Stefanie
    Differential expression of cytoskeletal-associated genes in human podocytes by the mTOR inhibitor everolimus
    In: Pediatric Nephrology Vol. 28 (2013) Nr. 8, pp. 1438 - 1438
  • Jeruschke, Stefanie; Büscher, Anja Katrin; Oh, Jun; Saleem, Moin Ahson; Hoyer, Peter Friedrich; Weber, Stefanie; Nalbant, Perihan
    Protective effects of the mTOR inhibitor everolimus on cytoskeletal injury in human podocytes are mediated by RhoA signaling
    In: PLoS ONE Vol. 8 (2013) Nr. 2, pp. e55980
    Online Full Text: dx.doi.org/ Online Full Text (Open Access)
  • Sokolova, Viktoriya; Rotan, Olga; Klesing, Jan; Nalbant, Perihan; Buer, Jan; Knuschke, Torben; Westendorf, Astrid; Epple, Matthias
    Calcium phosphate nanoparticles as versatile carrier for small and large molecules across cell membranes
    In: Journal of Nanoparticle Research Vol. 14 (2012) Nr. 6, 0910
    Online Full Text: dx.doi.org/
  • Jeruschke, Stefanie; Büscher (A.), Anja; Saleem, Moin; Hoyer, Peter Friedrich; Oh, Jun; Nalbant, Perihan; Weber, Stefanie
    Protective effects of everolimus on puromycin-induced cytoskeletal alterations in human podocytes are mediated by RhoA-signalling
    In: Pediatric Nephrology Vol. 27 (2012) Nr. 9, pp. 1638 - 1638
  • MacHacek, Matthias; Hodgson, Louis; Welch, Christopher; Elliott, Hunter; Pertz, Oliver; Nalbant, Perihan; Abell, Amy; Johnson, Gary L.; Hahn, Klaus M.; Danuser, Gaudenz
    Coordination of Rho GTPase activities during cell protrusion
    In: Nature Vol. 461 (2009) Nr. 7260, pp. 99 - 103
    Online Full Text
  • Nalbant, Perihan; Chang, Y.-C.; Birkenfeld, J.; Chang, Z.-F.; Bokoch, G.M.
    Guanine nucleotide exchange factor-H1 regulates cell migration via localized activation of RhoA at the leading edge
    In: Molecular Biology of the Cell Vol. 20 (2009) Nr. 18, pp. 4070 - 4082
  • Manukyan, Maria; Nalbant, Perihan; Luxen, Sylvia; Hahn, Klaus M.; Knaus, Ulla G.
    RhoA GTPase activation by TLR2 and TLR3 ligands: Connecting via Src to NF-κB
    In: The Journal of Immunology (JI) Vol. 182 (2009) Nr. 6, pp. 3522 - 3529
    Online Full Text: dx.doi.org/
  • Chang, Y.-C.; Nalbant, Perihan; Birkenfeld, J.; Chang, Z.-F.; Bokoch, G.M.
    3GEF-H1 couples nocodazole-induced microtubule disassembly to cell contractility via RhoA
    In: Molecular Biology of the Cell Vol. 19 (2008) Nr. 5, pp. 2147 - 2153
  • Birkenfeld, Jörg; Nalbant, Perihan; Yoon, Soon-Hee; Bokoch, Gary M.
    Cellular functions of GEF-H1, a microtubule-regulated Rho-GEF: is altered GEF-H1 activity a crucial determinant of disease pathogenesis?
    In: Trends in Cell Biology Vol. 18 (2008) Nr. 5, pp. 210 - 219
    Online Full Text
  • Carlile, Mark; Nalbant, Perihan; Preston-Fayers, Keziah; McHaffie, Gavin S.; Werner, Andreas
    Processing of naturally occurring sense/antisense transcripts of the vertebrate Slc34a gene into short RNAs
    In: Physiological Genomics Vol. 34 (2008) Nr. 1, pp. 95 - 100
    Online Full Text
  • Lim, Yangmi; Lim, Ssang-Taek; Tomar, Alok; Gardel, Margaret; Bernard-Trifilo, Joie A.; Xiao, Lei Chen; Uryu, Sean A.; Canete-Soler, Rafaela; Zha, Jinbin; Lin, Hong; Schlaepfer, William W.; Nalbant, Perihan; Bokoch, Gary; Ilic, Dusko; Waterman-Storer, Clare; Schlaepfer, David D.
    PyK2 and FAK connections to p190Rho guanine nucleotide exchange factor regulate RhoA activity, focal adhesion formation, and cell motility
    In: The Journal of Cell Biology (JCB) Vol. 180 (2008) Nr. 1, pp. 187 - 203
    Online Full Text: dx.doi.org/
  • El-Sibai, Mirvat; Nalbant, Perihan; Pang, Huan; Flinn, Rory J.; Sarmiento, Corina; Macaluso, Frank; Cammer, Michael; Condeelis, John S.; Hahn, Klaus M.; Backer, Jonathan M.
    Cdc42 is required for EGF-stimulated protrusion and motility in MTLn3 carcinoma cells
    In: Journal of Cell Science Vol. 120 (2007) Nr. 19, pp. 3465 - 3474
    Online Full Text
  • Birkenfeld, Jörg; Nalbant, Perihan; Bohl, Benjamin P.; Pertz, Oliver; Hahn, Klaus M.; Bokoch, Gary M.
    GEF-H1 Modulates Localized RhoA Activation during Cytokinesis under the Control of Mitotic Kinases
    In: Developmental Cell Vol. 12 (2007) Nr. 5, pp. 699 - 712
    Online Full Text: dx.doi.org/ (Open Access)
  • Frantz, Christian; Karydis, Anastasios; Nalbant, Perihan; Hahn, Klaus M.; Barber, Diane L.
    Positive feedback between Cdc42 activity and H+ efflux by the Na-H exchanger NHE1 for polarity of migrating cells
    In: The Journal of Cell Biology (JCB) Vol. 179 (2007) Nr. 3, pp. 403 - 410
    Online Full Text
  • Zhao, Tieming; Nalbant, Perihan; Hoshino, Mikio; Dong, Xuemei; Wu, Dianqing; Bokoch, Gary M.
    Signaling requirements for translocation of P-Rex1, a key Rac2 exchange factor involved in chemoattractant-stimulated human neutrophil function
    In: Journal of Leukocyte Biology Vol. 81 (2007) Nr. 4, pp. 1127 - 1136
    Online Full Text
  • Manukyan, Maria; Nalbant, Perihan; Pertz, Oliver; Halut, Klaus; Knaus, Ulla G.
    Visualizing TLR2- and TLR3-dependent RhoA activation by fret biosensor
    In: Journal of Leukocyte Biology (2007) Nr. Supplement, pp. 36 - 36
  • Dehmelt, Leif; Nalbant, Perihan; Steffen, Walter; Halpain, Shelley
    A microtubule-based, dynein-dependent force induces local cell protrusions: Implications for neurite initiation
    In: Brain Cell Biology Vol. 35 (2006) Nr. 1, pp. 39 - 56
  • Hodgson, Louis; Nalbant, Perihan; Shen, Feimo; Hahn, Klaus M.
    Imaging and photobleach correction of Mero-CBD, sensor of endogenous Cdc42 activation
    In: Methods in Enzymology Vol. 406 (2006) Nr. 12, pp. 140 - 156
  • Belvindrah, R.; Nalbant, Perihan; Ding, S.; Wu, C.; Bokoch, Gary M.; Müller, U.
    Integrin-linked kinase regulates Bergmann glial differentiation during cerebellar development
    In: Molecular and Cellular Neuroscience (MCN) Vol. 33 (2006) Nr. 2, pp. 109 - 125
  • Subauste, M. Cecilia; Nalbant, Perihan; Adamson, Eileen D.; Hahn, Klaus M.
    Vinculin controls PTEN protein level by maintaining the interaction of the adherens junction protein β-catenin with the scaffolding protein MAGI-2
    In: The Journal of Biological Chemistry (JBC) Vol. 280 (2005) Nr. 7, pp. 5676 - 5681
    Online Full Text
  • Nalbant, Perihan; Hodgson, Louis; Kraynov, Vadim; Toutchkine, Alexei; Hahn, Klaus M.
    Activation of endogenous Cdc42 visualized in living cells
    In: Science Vol. 305 (2004) Nr. 5690, pp. 1615 - 1619
  • Tan, Xin; Nalbant, Perihan; Toutchkine, Alexei; Hu, Dehong; Vorpagel, Erich R.; Hahn, Klaus M.; Lu, H. Peter
    Single-Molecule Study of Protein-Protein Interaction Dynamics in a Cell Signaling System
    In: Journal of Physical Chemistry B: Condensed Matter, Materials, Surfaces, Interfaces & Biophysical Chemistry Vol. 108 (2004) Nr. 2, pp. 737 - 744
    Online Full Text
  • Graham, C.; Nalbant, Perihan; Schölermann, B.; Hentschel, H.; Kinne, R. K. H.; Werner, A.
    Characterization of a type IIb sodium-phosphate cotransporter from zebrafish (Danio rerio) kidney
    In: American Journal of Physiology - Renal Physiology Vol. 284 (2003) Nr. 4, pp. F727 - F736
    Online Full Text
  • Tan, Xin; Nalbant, Perihan; Hu, Dehong; Vorpagel, Erich R.; Hahn, Klaus M.; Lu, H. Peter
    Single-molecule protein-protein interaction dynamics of GTP-binding protein Cdc42 with downstream effector WASP
    In: Biophysical Journal Vol. 84 (2003) Nr. Suppl 2 Pt 2, pp. 485a
    Online Full Text
  • Toutchkine, Alexei; Nalbant, Perihan; Hahn, Klaus M.
    Facile synthesis of thiol-reactive Cy3 and Cy5 derivatives with enhanced water solubility
    In: Bioconjugate Chemistry Vol. 13 (2002) Nr. 3, pp. 387 - 391
    Online Full Text
  • Werner, A.; Preston-Fayers, K.; Dehmelt, L.; Nalbant, Perihan
    Regulation of the NPT gene by a naturally occurring antisense transcript
    In: Cell Biochemistry and Biophysics Vol. 36 (2002) Nr. 2/3, pp. 241 - 252
  • Nalbant, Perihan; Boehmer, Christoph; Dehmelt, Leif; Wehner, Frank; Werner, Andreas
    Functional characterization of a Na+-phosphate cotransporter (NaP(i)-II) from zebrafish and identification of related transcripts
    In: Journal of Physiology Vol. 520 (1999) Nr. 1, pp. 79 - 89
    Online Full Text
  • Werner, Andreas; Dehmelt, Leif; Nalbant, Perihan
    Na+-dependent phosphate cotransporters : The NaPi protein families
    In: The Journal of Experimental Biology Vol. 201 (1998) Nr. 23, pp. 3135 - 3142
Read more