ZMB Member Farnusch Kaschani
ZMB Member
Farnusch Kaschani
Next ZMB-Member
Dr. Farnusch Kaschani
Group Chemical Proteomics
Analytics Core Facility Essen (ACE)Center of Medical Biotechnology (ZMB)
University of Duisburg-Essen
Universitätsstr. 2
45141 Essen
- +49 201 183 4924
- Website
- Selected Publications
- ZMB Research Program
Molecular and Chemical Cell Biology
Research Overview
The Analytics Core Facility Essen (ACE) is a central facility of the Center of Medical Biotechnology (ZMB) and of the Faculty of Biology of the University Duisburg-Essen. It offers access to state-of-the-art mass spectrometry-based proteomics for internal and external users. The timely technical equipment of the ACE allows us to provide a wide variety of different “bottom-up” and “top-down” proteomics methods. Furthermore, we also develop innovative solutions for project-specific demands. The service comprises all aspects of mass spectrometry-based proteomics, starting from technical advice and project planning, sample preparation and analysis to data analysis, data management and interpretation of results. We are also listed in the RIsources Poral of the DFG (RI_00466).
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Selected Publications
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Activity-based proteomics uncovers suppressed hydrolases and a neo-functionalised antibacterial enzyme at the plant–pathogen interfaceIn: New Phytologist Vol. 241 (2024) Nr. 1, pp. 394 - 408Online Full Text: dx.doi.org/ (Open Access)
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Characterization of 2-phenanthroate:CoA ligase from the sulfate-reducing, phenanthrene-degrading enrichment culture TRIPIn: Applied and Environmental Microbiology Vol. 90 (2024) Nr. 10, e0129624Online Full Text: dx.doi.org/ (Open Access)
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Differences in the Synovial Fluid Proteome of Septic and Aseptic Implant FailureIn: Antibiotics Vol. 13 (2024) Nr. 4, 346Online Full Text: dx.doi.org/ Online Full Text (Open Access)
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Discovery of active mouse, plant and fungal cytochrome P450s in endogenous proteomes and upon expression in plantaIn: Scientific Reports Vol. 14 (2024) Nr. 1, 10091Online Full Text: dx.doi.org/ (Open Access)
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Environmental activity-based protein profiling for function-driven enzyme discovery from natural communitiesIn: Environmental Microbiomes Vol. 19 (2024) Nr. 1, 36Online Full Text: dx.doi.org/ (Open Access)
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Extracellular plant subtilases dampen cold-shock peptide elicitor levelsIn: Nature Plants Vol. 10 (2024) Nr. 11, pp. 1749 - 1760Online Full Text: dx.doi.org/ (Open Access)
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Factors governing attachment of Rhizobium leguminosarum to legume roots at acid, neutral, and alkaline pHsIn: mSystems Vol. 9 (2024) Nr. 9, e0042224Online Full Text: dx.doi.org/ (Open Access)
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Glutathione Transferase Photoaffinity Labeling Displays GST Induction by Safeners and Pathogen InfectionIn: Plant and Cell Physiology Vol. 65 (2024) Nr. 1, pp. 128 - 141Online Full Text: dx.doi.org/ (Open Access)
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High resolution analysis of proteolytic substrate processingIn: The Journal of Biological Chemistry (JBC) Vol. 300 (2024) Nr. 11, 107812Online Full Text: dx.doi.org/ (Open Access)
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Induced degradation of SNAP-fusion proteinsIn: RSC Chemical Biology Vol. 5 (2024) Nr. 12, pp. 1232 - 1247Online Full Text: dx.doi.org/ (Open Access)
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Microtubule end-on attachment maturation regulates Mps1 association with its kinetochore receptorIn: Current Biology Vol. 34 (2024) Nr. 11, pp. 2279 - 2293.e6Online Full Text: dx.doi.org/ (Open Access)
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Subtilase SBT5.2 inactivates flagellin immunogenicity in the plant apoplastIn: Nature Communications Vol. 15 (2024) Nr. 1, 10431Online Full Text: dx.doi.org/ (Open Access)
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The anti-tubercular callyaerins target the Mycobacterium tuberculosis-specific non-essential membrane protein Rv2113In: Cell Chemical Biology Vol. 31 (2024) Nr. 10, pp. 1755 - 1771.e73Online Full Text: dx.doi.org/ (Open Access)
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The proteome of Nicotiana benthamiana is shaped by extensive protein processingIn: New Phytologist Vol. 243 (2024) Nr. 3, pp. 1034 - 1049Online Full Text: dx.doi.org/ (Open Access)
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TopBP1 utilises a bipartite GINS binding mode to support genome replicationIn: Nature Communications Vol. 15 (2024) Nr. 1, 1797
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Chemoproteomics Reveals the Pan-HER Kinase Inhibitor Neratinib To Target an Arabidopsis Epoxide Hydrolase Related to Phytohormone SignalingIn: ACS Chemical Biology Vol. 18 (2023) Nr. 5, pp. 1076 - 1088Online Full Text: dx.doi.org/
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Clp-targeting BacPROTACs impair mycobacterial proteostasis and survivalIn: Cell Vol. 186 (2023) Nr. 10, pp. 2176 - 2192.e22Online Full Text: dx.doi.org/ (Open Access)
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IFNα primes cancer cells for Fusicoccin-induced cell death via 14-3-3 PPI stabilizationIn: Cell Chemical Biology Vol. 30 (2023) Nr. 6, pp. 573 - 590.e6Online Full Text: dx.doi.org/ Online Full Text (Open Access)
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Structure of the peroxisomal Pex1/Pex6 ATPase complex bound to a substrateIn: Nature Communications Vol. 14 (2023) Nr. 1, 5942Online Full Text: dx.doi.org/; Online Full Text: dx.doi.org/ (Open Access)
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Taspase1 facilitates Topoisomerase IIβ-mediated DNA double-strand breaks driving estrogen-induced transcriptionIn: Cells Vol. 2023 (2023) Nr. 12, 363Online Full Text: dx.doi.org/ Online Full Text (Open Access)
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The UBX domain in UBXD1 organizes ubiquitin binding at the C-terminus of the VCP/p97 AAA-ATPaseIn: Nature Communications Vol. 14 (2023) Nr. 1, 3258Online Full Text: dx.doi.org/ Online Full Text (Open Access)
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The root pathogen Aphanomyces euteiches secretes modular proteases in pea apoplast during host infectionIn: Frontiers in Plant Science (FPLS) Vol. 14 (2023) 1140101Online Full Text: dx.doi.org/ (Open Access)
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Activity-Based Protein Profiling for the Identification of Novel Carbohydrate-Active Enzymes Involved in Xylan Degradation in the Hyperthermophilic Euryarchaeon Thermococcus sp. Strain 2319x1EIn: Frontiers in Microbiology Vol. 12 (2022) 734039Online Full Text: dx.doi.org/ Online Full Text (Open Access)
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Erratum: Author Correction: Identification of fungal lignocellulose-degrading biocatalysts secreted by Phanerochaete chrysosporium via activity-based protein profiling (Communications biology (2022) 5 1 (1254))In: Communications Biology Vol. 5 (2022) Nr. 1, pp. 1319Online Full Text: dx.doi.org/ (Open Access)
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Identification of fungal lignocellulose-degrading biocatalysts secreted by Phanerochaete chrysosporium via activity-based protein profilingIn: Communications Biology Vol. 5 (2022) Nr. 1, 1254Online Full Text: dx.doi.org/ Online Full Text (Open Access)
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Persister state-directed transitioning and vulnerability in melanoma
32. Deutscher Hautkrebskongress (ADO-Jahrestagung), 14.–17. September 2022, Hannover, Germany,In: Nature Communications Vol. 13 (2022) Nr. 1, pp. 35 - 36 -
Article leaf apoplast of field-grown potato analyzed by quantitative proteomics and activity-based protein profilingIn: International Journal of Molecular Sciences (IJMS) Vol. 22 (2021) Nr. 21, 12033Online Full Text: dx.doi.org/ (Open Access)
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Correction to : Natural brominated phenoxyphenols kill persistent and biofilm-incorporated cells of MRSA and other pathogenic bacteriaIn: Applied Microbiology and Biotechnology Vol. 105 (2021) Nr. 14-15, pp. 6119 - 6119Online Full Text: dx.doi.org/ (Open Access)
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Statins affect cancer cell plasticity with distinct consequences for tumor progression and metastasisIn: Cell Reports Vol. 37 (2021) Nr. 8, 110056Online Full Text: dx.doi.org/ Online Full Text (Open Access)
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Targeted substrate loop insertion by VCP/p97 during PP1 complex disassemblyIn: Nature Structural & Molecular Biology Vol. 28 (2021) Nr. 12, pp. 964 - 971Online Full Text: dx.doi.org/
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The chemical compound ‘Heatin’ stimulates hypocotyl elongation and interferes with the Arabidopsis NIT1-subfamily of nitrilasesIn: The Plant Journal Vol. 106 (2021) Nr. 6, pp. 1523 - 1540Online Full Text: dx.doi.org/ (Open Access)
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Accelerated trypsin autolysis by affinity polymer templatesIn: RSC Advances Vol. 10 (2020) Nr. 48, pp. 28711 - 28719Online Full Text: dx.doi.org/ (Open Access)
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Accelerated trypsin autolysis by affinity polymer templatesIn: RSC Advances Vol. 10 (2020) Nr. 48, pp. 28711 - 28719Online Full Text: dx.doi.org/ (Open Access)
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Discovering the RNA-binding proteome of plant leaves with an improved RNA interactome capture methodIn: Biomolecules Vol. 10 (2020) Nr. 4, 661Online Full Text: dx.doi.org/ (Open Access)
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Natural brominated phenoxyphenols kill persistent and biofilm-incorporated cells of MRSA and other pathogenic bacteriaIn: Applied Microbiology and Biotechnology Vol. 104 (2020) Nr. 13, pp. 5985 - 5998Online Full Text: dx.doi.org/ (Open Access)
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Protein Phosphatase-1 Complex Disassembly by p97 is Initiated through Multivalent Recognition of Catalytic and Regulatory Subunits by the p97 SEP-domain AdaptersIn: Journal of Molecular Biology (JMB) Vol. 432 (2020) Nr. 23, pp. 6061 - 6074Online Full Text: dx.doi.org/
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Targeting of parvulin interactors by diazirine mediated cross-linking discloses a cellular role of human Par14/17 in actin polymerizationIn: Biological Chemistry Vol. 401 (2020) Nr. 8, pp. 955 - 968Online Full Text: dx.doi.org/ (Open Access)
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Using proteomics for an insight into the performance of activated sludge in a lab-scale WWTPIn: International Biodeterioration & Biodegradation Vol. 149 (2020) pp. 104934Online Full Text: dx.doi.org/
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Zelkovamycin is an OXPHOS Inhibitory Member of the Argyrin Natural Product FamilyIn: Chemistry - A European Journal Vol. 26 (2020) Nr. 39, pp. 8524 - 8531Online Full Text: dx.doi.org/ (Open Access)
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A homology-guided, genome-based proteome for improved proteomics in the alloploid Nicotiana benthamianaIn: BMC Genomics Vol. 20 (2019) Nr. 1, pp. 722Online Full Text: dx.doi.org/ (Open Access)
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Activity-based proteomics reveals nine target proteases for the recombinant protein-stabilizing inhibitor S/CYS8 in Nicotiana benthamianaIn: Plant Biotechnology Journal Vol. 17 (2019) Nr. 8, pp. 1670 - 1678Online Full Text: dx.doi.org/ (Open Access)
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Cathepsin L regulates metabolic networks controlling rapid cell growth and proliferationIn: Molecular and Cellular Proteomics (MCP) Vol. 18 (2019) Nr. 7, pp. 1330 - 1344Online Full Text: dx.doi.org/ (Open Access)
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Cathepsins Drive Anti-Inflammatory Activity by Regulating Autophagy and Mitochondrial Dynamics in Macrophage Foam CellsIn: Cellular Physiology and Biochemistry Vol. 53 (2019) Nr. 3, pp. 550 - 572Online Full Text: dx.doi.org/ (Open Access)
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Dynamic hydrolase labelling as a marker for seed quality in Arabidopsis seedsIn: Biochemical Journal Vol. 476 (2019) Nr. 5, pp. 843 - 857Online Full Text: dx.doi.org/ (Open Access)
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Glycosidase and glycan polymorphism control hydrolytic release of immunogenic flagellin peptidesIn: Science Vol. 364 (2019) Nr. 6436, eaav0748Online Full Text: dx.doi.org/ Online Full Text (Open Access)
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Metabolic reconstruction of the genome of candidate Desulfatiglans TRIP_1 and identification of key candidate enzymes for anaerobic phenanthrene degradationIn: Environmental Microbiology Vol. 21 (2019) Nr. 4, pp. 1267 - 1286Online Full Text: dx.doi.org/; Online Full Text: dx.doi.org/ (Open Access)
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Plant chemical genetics reveals colistin sulphate as a SA and NPR1-independent PR1 inducer functioning via a p38-like kinase pathwayIn: Scientific Reports Vol. 9 (2019) Nr. 1, pp. 11196Online Full Text: dx.doi.org/ (Open Access)
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Proteases underground : Analysis of the maize root apoplast identifies organ specific papain-like cysteine protease activityIn: Frontiers in Plant Science (FPLS) Vol. 10 (2019)Online Full Text: dx.doi.org/ (Open Access)
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Triazine probes target ascorbate peroxidases in plantsIn: Plant Physiology Vol. 180 (2019) Nr. 4, pp. 1848 - 1859Online Full Text: dx.doi.org/ (Open Access)
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A Non-Competitive Inhibitor of VCP/p97 and VPS4 Reveals Conserved Allosteric Circuits in Type I and II AAA ATPasesIn: Angewandte Chemie International Edition Vol. 57 (2018) Nr. 6, pp. 1576 - 1580Online Full Text: dx.doi.org/
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An apoplastic peptide activates salicylic acid signalling in maizeIn: Nature Plants Vol. 4 (2018) Nr. 3, pp. 172 - 180Online Full Text: dx.doi.org/ Online Full Text (Open Access)
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Fermentative Spirochaetes mediate necromass recycling in anoxic hydrocarbon-contaminated habitatsIn: ISME Journal Vol. 12 (2018) Nr. 8, pp. 2039 - 2050Online Full Text: dx.doi.org/; Online Full Text: dx.doi.org/ (Open Access)
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HTRA1-Dependent Cell Cycle ProteomicsIn: Journal of Proteome Research Vol. 17 (2018) Nr. 8, pp. 2679 - 2694Online Full Text: dx.doi.org/
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Identification of Noncatalytic Lysine Residues from Allosteric Circuits via Covalent ProbesIn: ACS Chemical Biology Vol. 13 (2018) Nr. 5, pp. 1307 - 1312Online Full Text: dx.doi.org/
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Identification of the Natural Product RotihibinA as a TOR Kinase Signaling Inhibitor by Unbiased Transcriptional ProfilingIn: Chemistry - A European Journal Vol. 24 (2018) Nr. 48, pp. 12500 - 12504Online Full Text: dx.doi.org/
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Low Protease Content in Medicago truncatula Cell Cultures Facilitates Recombinant Protein ProductionIn: Biotechnology Journal Vol. 13 (2018) Nr. 7, 1800050Online Full Text: dx.doi.org/
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Multiplex Fluorescent, Activity-Based Protein Profiling Identifies Active α-Glycosidases and Other Hydrolases in PlantsIn: Plant Physiology Vol. 177 (2018) Nr. 1, pp. 24 - 37Online Full Text: dx.doi.org/ (Open Access)
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Protease Activities Triggered by Ralstonia solanacearum Infection in Susceptible and Tolerant Tomato LinesIn: Molecular and Cellular Proteomics (MCP) Vol. 17 (2018) Nr. 6, pp. 1112 - 1125Online Full Text: dx.doi.org/ Online Full Text (Open Access)
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The transcriptome, extracellular proteome and active secretome of agroinfiltrated Nicotiana benthamiana uncover a large, diverse protease repertoireIn: Plant Biotechnology Journal Vol. 16 (2018) Nr. 5, pp. 1068 - 1084Online Full Text: dx.doi.org/ (Open Access)
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Three unrelated protease inhibitors enhance accumulation of pharmaceutical recombinant proteins in Nicotiana benthamianaIn: Plant Biotechnology Journal Vol. 16 (2018) Nr. 10, pp. 1797 - 1810Online Full Text: dx.doi.org/ (Open Access)
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Ubiquitin-Independent Disassembly by a p97 AAA-ATPase Complex Drives PP1 Holoenzyme FormationIn: Molecular Cell Vol. 72 (2018) Nr. 4, pp. 766 - 777Online Full Text: dx.doi.org/ (Open Access)
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Utilities for Mass Spectrometry Analysis of Proteins (UMSAP) : Fast post-processing of mass spectrometry dataIn: Rapid Communications in Mass Spectrometry Vol. 32 (2018) Nr. 19, pp. 1659 - 1667Online Full Text: dx.doi.org/
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Activity-based protein profiling as a robust method for enzyme identification and screening in extremophilic ArchaeaIn: Nature Communications Vol. 8 (2017) pp. 15352Online Full Text: dx.doi.org/ (Open Access)
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Mesenchymal stem cell therapy protects lungs from radiation-induced endothelial cell loss by restoring superoxide dismutase 1 expressionIn: Antioxidants and Redox Signaling Vol. 26 (2017) Nr. 11, pp. 563 - 582Online Full Text: dx.doi.org/ (Open Access)
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Proteasome activity profiling uncovers alteration of catalytic β2 and β5 subunits of the stress-induced proteasome during salinity stress in tomato rootsIn: Frontiers in Plant Science (FPLS) Vol. 8 (2017) 107Online Full Text: dx.doi.org/ (Open Access)
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Quantitative proteomics reveals ecophysiological effects of light and silver stress on the mixotrophic protist Poterioochromonas malhamensisIn: PLoS ONE Vol. 12 (2017) Nr. 1, pp. e0168183Online Full Text: dx.doi.org/ Online Full Text (Open Access)
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Subunit-selective proteasome activity profiling uncovers uncoupled proteasome subunit activities during bacterial infectionsIn: The Plant Journal Vol. 90 (2017) Nr. 2, pp. 418 - 430Online Full Text: dx.doi.org/
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The Root Growth-Regulating Brevicompanine Natural Products Modulate the Plant Circadian ClockIn: ACS Chemical Biology Vol. 12 (2017) Nr. 6, pp. 1466 - 1471Online Full Text: dx.doi.org/ (Open Access)
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Turning-ON ProteasomesIn: Cell Chemical Biology Vol. 24 (2017) Nr. 6, pp. 653 - 655Online Full Text: dx.doi.org/
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A novel quenched fluorescent activity-based probe reveals caspase-3 activity in the endoplasmic reticulum during apoptosisIn: Chemical Science Vol. 7 (2016) pp. 1322 - 1337Online Full Text: dx.doi.org/ (Open Access)
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Capture of endogenously biotinylated proteins from Pseudomonas aeruginosa displays unexpected downregulation of LiuD upon iron nutritionIn: Bioorganic and Medicinal Chemistry Vol. 24 (2016) Nr. 15, pp. 3330 - 3335Online Full Text: dx.doi.org/
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Screen of Non-annotated Small Secreted Proteins of Pseudomonas syringae Reveals a Virulence Factor That Inhibits Tomato Immune ProteasesIn: PLoS Pathogens Vol. 12 (2016) Nr. 9, pp. 1005874Online Full Text: dx.doi.org/ (Open Access)
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VCP/p97 Extracts Sterically Trapped Ku70/80 Rings from DNA in Double-Strand Break RepairIn: Molecular Cell Vol. 64 (2016) Nr. 1, pp. 189 - 198Online Full Text: dx.doi.org/ (Open Access)
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Chemical proteomics versus LeishmaniasisIn: Chemistry and Biology Vol. 22 (2015) Nr. 3, pp. 309 - 310Online Full Text: dx.doi.org/ (Open Access)
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Chemoproteomic Evaluation of the Polyacetylene Callyspongynic AcidIn: Chemistry - A European Journal Vol. 21 (2015) Nr. 30, pp. 10721 - 10728Online Full Text: dx.doi.org/ (Open Access)
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Determinants of amyloid fibril degradation by the PDZ protease HTRA1In: Nature Chemical Biology Vol. 11 (2015) Nr. 11, pp. 862 - 869Online Full Text: dx.doi.org/ Online Full Text (Open Access)
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Functional divergence of two secreted immune proteases of tomatoIn: Current Biology Vol. 25 (2015) Nr. 17, pp. 2300 - 2306Online Full Text: dx.doi.org/ (Open Access)