Apotome
Light MicroscopyZeiss Apotome
Technical Details:
Microscope frame: Zeiss AxioObserver.Z1 motorized inverted epifluorescence microscope.
Illumination with metal halide HXP-120 Lamp and Colibri high performance LED at 385nm, 430nm, 475nm, 555nm, 590nm, 630nm and 735nm.
monochrome Camera: Orca flash 16 bit data depth 4.2 Megapixel monochrome sCMOS camera.
color Camera: Axiocam 506 color 6 Megapixel 12x10mm Chipsize
Objektive:
|
Objektive Type |
Magnification |
Contrast | NA |
Working Distance (mm) |
Immersion |
|---|---|---|---|---|---|
|
EC Plan-Neofluar |
5 x |
- |
0.16 |
18.5 |
Air |
|
Plan-Apochromat |
10 x |
DIC |
0.45 |
2.0 |
Air |
|
N-Achroplan |
10 x |
PH1 |
0.25 |
6.0 |
Air |
|
Plan-Apochromat |
20 x |
DIC |
0.8 |
0.55 |
Air |
|
LD Plan-Neofluor Corr* |
20 x |
PH2 |
0.4 |
7.9 |
Air |
|
Plan-Apochromat |
40 x |
DIC |
0.95 |
0.25 |
Air |
|
LD C-Apochromat |
40 x |
DIC |
1.1 |
0.62 |
Water |
|
Plan-Apochromat |
63 x |
DIC |
1.4 |
0.19 |
Oil |
*upon request
filter Setup:
| Filter Set | Excitation | Dichroic | Emission | Fluorophores |
|---|---|---|---|---|
| Zeiss filter set 49 | G365 | 395 | BP 445/50 | DAPI |
|
Zeiss filter set 38 |
BP 470/40 | 495 | BP 525/50 | FITC, Alexa 488, EGFP |
|
Custom Filter Set |
BP 377/50 | 409 | BP 525/50 | eCFP, BV510 |
| AHF F56-429* |
DBP |
493 & 574 |
DBP 512/30 & 630/100 |
GFP & DsRed upon request |
| Zeiss filter set 43 | BP 545/25 | 570 | BP 605/70 | TRITC, Cy3, Alexa 546, DsRED |
| AHF Filter Set F46-306* | BP 580/25 | 600 | BP 625/30 |
AF 594 upon request |
| Zeiss filter set 50 | BP 640/30 | 660 | BP 690/50 | Cy5, Alexa 647 |
| AHF filter Set F36-751 | BP 735/28 | 757 | BP 809/81 | Cy7, APC/Fire |
Software: ZEN Blue 2012
Live Cell Imaging Accessories: Pecon Large Chamber Incubation System S, comprising:
- Incubator and heating unit XL multi S1 (large heated chamber around inverted microscope)
- Temp module S.
- CO2 module S.
- Heated insert PS1 with CO2 cover PMS1.
- Heated insert P Lab-Tek with CO2 cover.
Zeiss ApoTome:
The Zeiss ApoTome3 uses structured illumination giving wide field optical sectioning to remove blur arising from out of focus fluorescence signal. The Zeiss Campus webpages show the ApoTome principle and operation.