ZMB Member Dominik Boos

ZMB Member
Dominik Boos

Next ZMB-Member
portrait photo of ZMB member Dominik Boos
© UDE/D.Boos

Prof. Dr. Dominik Boos

Group

Molecular Genetics II

Faculty of Biology
University of Duisburg-Essen
Universitätsstr. 2
45141 Essen

Research Overview

The lab will investigate principles of DNA replication in vertebrate cells. Complete and accurate replication of the genome is essential for life because it ensures the homeostasis of the genetic information during cell divisions. Although DNA replication has been extensively studied in yeast we know surprisingly little in vertebrates. Hence, we aim to advance our understanding how DNA replication occurs in vertebrates, how it is integrated into the cellular context, and how it is linked to genome instability and cancer.

In the next years the lab will investigate the initiation step of replication because it is a major focus of regulation of DNA replication. The lab will concentrate mainly on the Treslin-TopBP1-MTBP protein complex, which we demonstrated during my postdoc time in John Diffley’s lab to be a major regulation platform of initiation. This puts us into the position for the first time to unravel initiation processes from the perspective of one of the main players involved, Treslin-TopBP1-MTBP.

The 2013 NRW Rückkehrerprogramm award together with the interdisciplinary environment at the ZMB will allow me to build a lab offering internationally competitive research conditions for several lab members. Positions for genuinely interested PhD applicants and postdocs are available.
An affiliation of PhD students with the International Max-Planck Research School (IMPRS) might be possible.

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Publications

    Journal articles

  • Day, Matthew; Tetik, Bilal; Parlak, Milena; Almeida-Hernández, Yasser; Räschle, Markus; Kaschani, Farnusch; Siegert, Heike; Marko, Anika; Sanchez-Garcia, Elsa; Kaiser, Markus; Barker, Isabel A.; Pearl, Laurence H.; Oliver, Antony W.; Boos, Dominik
    TopBP1 utilises a bipartite GINS binding mode to support genome replication
    In: Nature Communications Vol. 15 (2024) Nr. 1, 1797
    Online Full Text: dx.doi.org/; Online Full Text: dx.doi.org/ (Open Access)
  • Caballero, Madison; Boos, Dominik; Koren, Amnon
    Cell-type specificity of the human mutation landscape with respect to DNA replication dynamics
    In: Cell Genomics Vol. 3 (2023) Nr. 6, 100315
    Online Full Text: dx.doi.org/ (Open Access)
  • Ferreira, Pedro; Sanchez-Pulido, Luis; Marko, Anika; Ponting, Chris P.; Boos, Dominik
    Refining the domain architecture model of the replication origin firing factor Treslin/TICRR
    In: Life Science Alliance Vol. 5 (2022) Nr. 5, e202101088
    Online Full Text: dx.doi.org/ (Open Access)
  • Zaffar, Eman; Ferreira, Pedro; Sanchez-Pulido, Luis; Boos, Dominik
    The Role of MTBP as a Replication Origin Firing Factor
    In: Biology Vol. 11 (2022) Nr. 6, 827
    Online Full Text: dx.doi.org/ Online Full Text (Open Access)
  • Zonderland, Gijs; Vanzo, Riccardo; Amitash, Sampath; Martín-Doncel, Elena; Coscia, Fabian; Mund, Andreas; Lerdrup, Mads; Benada, Jan; Boos, Dominik; Toledo, Luis
    The TRESLIN-MTBP complex couples completion of DNA replication with S/G2 transition
    In: Molecular Cell Vol. 82 (2022) Nr. 18, pp. 3350 - 3365.e7
    Online Full Text: dx.doi.org/ (Open Access)
  • Ferreira, Pedro; Höfer, Verena; Kronshage, Nora; Marko, Anika; Reusswig, Karl-Uwe; Tetik, Bilal; Dießel, Christoph; Köhler, Kerstin; Tschernoster, Nikolai; Altmüller, Janine; Schulze, Nina; Pfander, Boris; Boos, Dominik
    MTBP phosphorylation controls DNA replication origin firing
    In: Scientific Reports Vol. 11 (2021) Nr. 1, 4242
    Online Full Text: dx.doi.org/ Online Full Text (Open Access)
  • Boos, Dominik; Ferreira, Pedro
    Origin firing regulations to control genome replication timing
    In: Genes Vol. 10 (2019) Nr. 3, pp. 199
    Online Full Text: dx.doi.org/ (Open Access)
  • Köhler, Kerstin; Sanchez-Pulido, Luis; Höfer, Verena; Marko, Anika; Ponting, Chris P.; Snijders, Ambrosius P.; Feederle, Regina; Schepers, Aloys; Boos, Dominik
    The Cdk8/19-cyclin C transcription regulator functions in genome replication through metazoan Sld7
    In: PLoS Biology Vol. 17 (2019) Nr. 1, e2006767
    Online Full Text: dx.doi.org/ (Open Access)
  • Day, Matthew; Rappas, Mathieu; Ptasinska, Katie; Boos, Dominik; Oliver, Antony W.; Pearl, Laurence H.
    BRCT domains of the DNA damage checkpoint proteins TOPBP1/Rad4 display distinct specificities for phosphopeptide ligands
    In: eLife Vol. 7 (2018) pp. e39979
    Online Full Text: dx.doi.org/ (Open Access)
  • Bantele, Susanne; Pfander, Dominik; Boos, Dominik; Ferreira, Pedro; Gritenaite, Dalia
    Targeting of the Fun30 nucleosome remodeller by the Dpb11 scaffold facilitates cell cycle-regulated DNA end resection
    In: eLife (2017) Nr. 6, pp. e21687
    Online Full Text: dx.doi.org/ Online Full Text (Open Access)
  • Boos, Dominik; Diffley, John F. X.; Yekezare, Mona
    Identification of a Heteromeric Complex That Promotes DNA Replication Origin Firing in Human Cells
    In: Science Vol. 340 (2013) Nr. 6135, pp. 981 - 984
    Online Full Text: dx.doi.org/
  • Boos, Dominik; Diffley, John FX; Frigola, Jordi
    Activation of the replicative DNA helicase : breaking up is hard to do
    In: Current Opinion in Cell Biology Vol. 24 (2012) Nr. 3, pp. 423 - 430
    Online Full Text: dx.doi.org/
  • Schöckel, Laura; Stemmann, Olaf; Boos, Dominik; Mayer, Bernd; Möckel, Martin
    Cleavage of cohesin rings coordinates the separation of centrioles and chromatids
    In: Nature Cell Biology Vol. 13 (2011) Nr. 8, pp. 966 - 972
    Online Full Text: dx.doi.org/
  • Boos, Dominik; Ponting, Chris P.; Oliver, Antony W.; Pearl, Laurence H.; Rappas, Mathieu; Sanchez-Pulido, Luis
    Regulation of DNA Replication through Sld3-Dpb11 Interaction Is Conserved from Yeast to Humans
    In: Current Biology Vol. 21 (2011) Nr. 13, pp. 1152 - 1157
    Online Full Text: dx.doi.org/
  • Boos, Dominik; Stemmann, Olaf; Kuffer, Christian; Lenobel, Rene
    Phosphorylation-dependent Binding of Cyclin B1 to a Cdc6-like Domain of Human Separase
    In: The Journal of Biological Chemistry (JBC) Vol. 283 (2008) Nr. 2, pp. 816 - 823
    Online Full Text: dx.doi.org/
  • Stemmann, Olaf; Boos, Dominik; Gorr, Ingo H.
    Anaphase Topsy-Turvy : Cdk1 a Securin, Separase a CKI
    In: Cell Cycle Vol. 5 (2006) Nr. 1, pp. 11 - 13
    Online Full Text: dx.doi.org/
  • Gorr, Ingo H.; Stemmann, Olaf; Wühr, Martin; Boos, Dominik; Reis, Alexandra
    Essential CDK1-inhibitory role for separase during meiosis I in vertebrate oocytes
    In: Nature Cell Biology Vol. 8 (2006) Nr. 9, pp. 1035 - 1037
    Online Full Text: dx.doi.org/
  • Gorr, Ingo H.; Boos, Dominik; Stemmann, Olaf
    Mutual inhibition of separase and Cdk1 by two-step complex formation
    In: Molecular Cell Vol. 19 (2005) Nr. 1, pp. 135 - 141
    Online Full Text: dx.doi.org/ (Open Access)
  • Stemmann, Olaf; Boos, Dominik; Gorr, Ingo H.
    Rephrasing anaphase : separase FEARs shugoshin
    In: Chromosoma Vol. 113 (2005) Nr. 8, pp. 409 - 417
    Online Full Text: dx.doi.org/