ZMB Member Bernd Giebel

ZMB Member
Bernd Giebel

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Bernd Giebel portrait
© UDE/Bettina Engel-Albustin

Prof. Dr. Bernd Giebel

Giebel Lab

Institute for Transfusion Medicine
University Hospital Essen
Hufelandstraße 55
45122 Essen

Research Overview

The group of Bernd Giebel investigates lineage relationships of early human hematopoietic progenitors as well as mechanisms controlling their self-renewal and lineage specification. In this context the group is especially interested in dissecting roles of asymmetric cell division, which they have proven to occur in at least a proportion of the cells. Since 2009, as a second topic the group is also working in the fast expanding field of extracellular vesicles (EVs), often termed exosomes or microvesicles, respectively. Here, the group is especially interested in deciphering the therapeutic potential of EVs released from mesenchymal stem/stromal cells (MSCs). After demonstrating therapeutic potentials in different systems including the treatment of a graft-versus-host disease patient, the group now aims to optimize and qualify the MSC-EV production for the clinical setting.
Keywords: Hematopoietic stem cells, hematopoiesis, mesenchymal stem cells, extracellular vesicles, exosomes
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Selected Publications

    Journal articles

  • Mouloud, Yanis; Staubach, Simon; Stambouli, Oumaima; Mokhtari, Shakiba; Kutzner, Tanja J; Zwanziger, Denise; Hemeda, Hatim; Giebel, Bernd
    Calcium chloride declotted human platelet lysate promotes the expansion of mesenchymal stromal cells and allows manufacturing of immunomodulatory active extracellular vesicle products
    In: Cytotherapy Vol. 26 (2024) Nr. 9, pp. 988 - 998
    Online Full Text: dx.doi.org/ (Open Access)
  • Barekzai, Jan; Refflinghaus, Laura; Okpara, Maduwuike; Tasto, Lars; Tertel, Tobias; Giebel, Bernd; Czermak, Peter; Salzig, Denise
    Process development for the production of mesenchymal stromal cell-derived extracellular vesicles in conventional 2D systems
    In: Cytotherapy Vol. 26 (2024) Nr. 9, pp. 999 - 1012
    Online Full Text: dx.doi.org/ (Open Access)
  • Toh, Wei Seong; Yarani, Reza; El Andaloussi, Samir; Cho, Byong Seung; Choi, Chulhee; Corteling, Randolph; De Fougerolles, Antonin; Gimona, Mario; Herz, Josephine; Khoury, Maroun; Robbins, Paul D.; Williams, Douglas; Weiss, Daniel J.; Rohde, Eva; Giebel, Bernd; Lim, Sai Kiang
    A report on the International Society for Cell & Gene Therapy 2022 Scientific Signature Series, “Therapeutic advances with native and engineered human extracellular vesicles”
    In: Cytotherapy Vol. 25 (2023) Nr. 8, pp. 810 - 814
    Online Full Text: dx.doi.org/
  • Bauer, Fabiola Nardi; Tertel, Tobias; Stambouli, Oumaima; Wang, Chen; Dittrich, Robin; Staubach, Simon; Börger, Verena; Hermann, Dirk M.; Brandau, Sven; Giebel, Bernd
    CD73 activity of mesenchymal stromal cell-derived extracellular vesicle preparations is detergent-resistant and does not correlate with immunomodulatory capabilities
    In: Cytotherapy Vol. 25 (2023) Nr. 2, pp. 138 - 147
    Online Full Text: dx.doi.org/ (Open Access)
  • Madel, Rabea J.; Börger, Verena; Dittrich, Robin; Bremer, Michel; Tertel, Tobias; Phuong, Nhi Ngo Thi; Baba, Hideo Andreas; Kordelas, Lambros; Staubach, Simon; Stein, Frank; Haberkant, Per; Hackl, Matthias; Grillari, Regina; Grillari, Johannes; Buer, Jan; Horn, Peter; Westendorf, Astrid; Brandau, Sven; Kirschning, Carsten Jürgen; Giebel, Bernd
    Independent human mesenchymal stromal cell–derived extracellular vesicle preparations differentially attenuate symptoms in an advanced murine graft-versus-host disease model
    In: Cytotherapy (2023)
    Online Full Text: dx.doi.org/ (Open Access)
  • Bremer, Michel; Nardi Bauer, Fabiola; Tertel, Tobias; Dittrich, Robin; Horn, Peter; Börger, Verena; Giebel, Bernd
    Qualification of a multidonor mixed lymphocyte reaction assay for the functional characterization of immunomodulatory extracellular vesicles
    In: Cytotherapy Vol. 25 (2023) Nr. 8, pp. 847 - 857
    Online Full Text: dx.doi.org/
  • Tertel, Tobias; Schoppet, Melanie; Stambouli, Oumaima; Al-Jipouri, Ali; James, Patrick F.; Giebel, Bernd
    Imaging flow cytometry challenges the usefulness of classically used extracellular vesicle labeling dyes and qualifies the novel dye Exoria for the labeling of mesenchymal stromal cell–extracellular vesicle preparations
    In: Cytotherapy Vol. 24 (2022) Nr. 6, pp. 619 - 628
    Online Full Text: dx.doi.org/ (Open Access)
  • Wang, Chen; Börger, Verena; Mohamud Yusuf, Ayan; Tertel, Tobias; Stambouli, Oumaima; Murke, Florian; Freund, Nico; Kleinschnitz, Christoph; Herz, Josephine; Gunzer, Matthias; Popa-Wagner, Aurel; Döppner, Thorsten R.; Giebel, Bernd; Hermann, Dirk M.
    Postischemic Neuroprotection Associated With Anti-Inflammatory Effects by Mesenchymal Stromal Cell-Derived Small Extracellular Vesicles in Aged Mice
    In: Stroke Vol. 53 (2022) Nr. 1, pp. E14 - E18
    Online Full Text: dx.doi.org/ (Open Access)
  • Hermann, Dirk M.; Döppner, Thorsten R.; Giebel, Bernd
    Circulating MicroRNAs : Posttranscriptional Regulators and Disease Markers Holding Promise in Stroke Prediction
    In: Stroke Vol. 52 (2021) Nr. 3, pp. 954 - 956
    Online Full Text: dx.doi.org/ (Open Access)
  • Gimona, Mario; Brizzi, Maria Felice; Choo, Andre Boon Hwa; Dominici, Massimo; Davidson, Sean M.; Grillari, Johannes; Hermann, Dirk M.; Hill, Andrew F.; de Kleijn, Dominique; Lai, Ruenn Chai; Lai, Charles P.; Lim, Rebecca; Monguió-Tortajada, Marta; Muraca, Maurizio; Ochiya, Takahiro; Ortiz, Luis A.; Toh, Wei Seong; Yi, Yong Weon; Witwer, Kenneth W.; Giebel, Bernd; Lim, Sai Kiang
    Critical considerations for the development of potency tests for therapeutic applications of mesenchymal stromal cell-derived small extracellular vesicles
    In: Cytotherapy Vol. 23 (2021) Nr. 5, pp. 373 - 380
    Online Full Text: dx.doi.org/ Online Full Text (Open Access)
  • Stambouli, O.; Dittrich, Robin; Nardi-Bauer, Fabiola; Tertel, Tobias; Horn, Peter; Giebel, Bernd
    Impact of mesenchymal stromal/stem cell isolation strategies on the immunomodulatory activity of their extracellular vesicles
    In: Cytotherapy Vol. 23 (2021) Nr. Suppl. 5, pp. 113
  • Hermann, Dirk M.; Döppner, Thorsten R.; Giebel, Bernd
    New Light on the Horizon : Extracellular Vesicles as Diagnostic Tool in Transient Ischemic Attack and Ischemic Stroke
    In: Stroke Vol. 52 (2021) Nr. 10, pp. 3348 - 3350
    Online Full Text: dx.doi.org/ (Open Access)
  • Börger, Verena; Weiss, Daniel J.; Anderson, Johnathon D.; Borràs, Francesc E.; Bussolati, Benedetta; Carter, David R.F.; Dominici, Massimo; Falcón-Pérez, Juan M.; Gimona, Mario; Hill, Andrew F.; Hoffman, Andrew M.; de Kleijn, Dominique; Levine, Bruce L.; Lim, Rebecca; Lötvall, Jan; Mitsialis, S. Alex; Monguió-Tortajada, Marta; Muraca, Maurizio; Nieuwland, Rienk; Nowocin, Anna; O'Driscoll, Lorraine; Ortiz, Luis A.; Phinney, Donald G; Reischl, Ilona; Rohde, Eva; Sanzenbacher, Ralf; Théry, Clotilde; Toh, Wei Seong; Witwer, Kenneth W.; Lim, Sai Kiang; Giebel, Bernd
    International Society for Extracellular Vesicles and International Society for Cell and Gene Therapy statement on extracellular vesicles from mesenchymal stromal cells and other cells : considerations for potential therapeutic agents to suppress coronavirus disease-19
    In: Cytotherapy Vol. 22 (2020) Nr. 9, pp. 482 - 485
    Online Full Text: dx.doi.org/ (Open Access)
  • Wang, Chen; Börger, Verena; Sardari, Maryam; Murke, Florian; Skuljec, Jelena; Pul, Refik; Hagemann, Nina; Dzyubenko, Egor; Dittrich, Robin; Gregorius, Jonas; Hasenberg, Mike; Kleinschnitz, Christoph; Popa-Wagner, Aurel; Döppner, Thorsten R.; Gunzer, Matthias; Giebel, Bernd; Hermann, Dirk M.
    Mesenchymal Stromal Cell-Derived Small Extracellular Vesicles Induce Ischemic Neuroprotection by Modulating Leukocytes and Specifically Neutrophils
    In: Stroke Vol. 9 (2020) Nr. 10, pp. 1825 - 1834
    Online Full Text: dx.doi.org/ (Open Access)
  • Sokolova, Viktoriya; Nzou, Goodwell; van der Meer, Selina; Ruks, Tatjana; Heggen, Marc; Loza, Kateryna; Hagemann, Nina; Murke, Florian; Giebel, Bernd; Hermann, Dirk M.; Atala, Anthony J.; Epple, Matthias
    Ultrasmall gold nanoparticles (2 nm) can penetrate and enter cell nuclei in an in vitro 3D brain spheroid model
    In: Acta Biomaterialia Vol. 111 (2020) pp. 349 - 362
    Online Full Text: dx.doi.org/
  • Bremer, M.; Börger, Verena; Horn, Peter; Giebel, Bernd
    A mixed lymphocyte reaction as a functional assay for extracellular vesicles of different origins
    In: Cytotherapy Vol. 21 (2019) Nr. 5, pp. 57
    Online Full Text: dx.doi.org/
  • Staubach, S.; Reiter, C.; Weber, G.; Giebel, Bernd
    Free flow electrophoresis allows preparation of extracellular vesicles with high purity
    In: Cytotherapy Vol. 21 (2019) Nr. Suppl. 5, pp. 57 - S58
    Online Full Text: dx.doi.org/
  • Madel, R.; Borger, V.; Bremer, M.; Horn, Peter; Baba, Hideo Andreas; Brandau, Sven; Buer, Jan; Giebel, Bernd; Kirschning, Carsten Jürgen
    MSC-EVS protect mice from graft-versus-host disease pathology in a preparation dependent manner
    In: Cytotherapy Vol. 21 (2019) Nr. Suppl. 5, pp. 57
    Online Full Text: dx.doi.org/
  • Börger, Verena; Dittrich, Ralf; Staubach, S.; Zumegen, S.; Horn, Peter; Giebel, Bernd
    Tangential flow filtration, a potential method for the scaled preparation of extracellular vesicles
    In: Cytotherapy Vol. 21 (2019) Nr. Suppl. 5, pp. 57
    Online Full Text: dx.doi.org/
  • Van Deun, Jan; Mestdagh, Pieter; Agostinis, Patrizia; Akay, Özden; Anand, Sushma; Anckaert, Jasper; Martinez, Zoraida Andreu; Baetens, Tine; Beghein, Els; Bertier, Laurence; Berx, Geert; Boere, Janneke; Boukouris, Stephanie; Bremer, Michel; Buschmann, Dominik; Byrd, James B.; Casert, Clara; Cheng, Lesley; Cmoch, Anna; Daveloose, Delphine; De Smedt, Eva; Demirsoy, Seyma; Depoorter, Victoria; Dhondt, Bert; Driedonks, Tom A.P.; Dudek, Aleksandra; Elsharawy, Abdou; Floris, Ilaria; Foers, Andrew D.; Gärtner, Kathrin; Garg, Abhishek D.; Geeurickx, Edward; Gettemans, Jan; Ghazavi, Farzaneh; Giebel, Bernd; Kormelink, Tom Groot; Hancock, Grace; Helsmoortel, Hetty; Hill, Andrew F.; Hyenne, Vincent; Kalra, Hina; Kim, David; Kowal, Joanna; Kraemer, Sandra; Leidinger, Petra; Leonelli, Carina; Liang, Yaxuan; Lippens, Lien; Liu, Shu; Lo Cicero, Alessandra; Martin, Shaun; Mathivanan, Suresh; Mathiyalagan, Prabhu; Matusek, Támas; Milani, Gloria; Monguió-Tortajada, Marta; Mus, Liselot M.; Muth, Dillon C.; Németh, Andrea; Nolte-'T Hoen, Esther N.M.; O'Driscoll, Lorraine; Palmulli, Roberta; Pfaffl, Michael W.; Primdal-Bengtson, Bjarke; Romano, Erminia; Rousseau, Quentin; Sahoo, Susmita; Sampaio, Natalia; Samuel, Monisha; Scicluna, Benjamin; Soen, Bieke; Steels, Anneleen; Swinnen, Johannes V.; Takatalo, Maarit; Thaminy, Safia; Théry, Clotilde; Tulkens, Joeri; Van Audenhove, Isabel; Van Der Grein, Susanne; Van Goethem, Alan; Van Herwijnen, Martijn J.; Van Niel, Guillaume; Van Roy, Nadine; Van Vliet, Alexander R.; Vandamme, Niels; Vanhauwaert, Suzanne; Vergauwen, Glenn; Verweij, Frederik; Wallaert, Annelynn; Wauben, Marca; Witwer, Kenneth W.; Zonneveld, Marijke I.; De Wever, Olivier; Vandesompele, Jo; Hendrix, An
    EV-TRACK : Transparent reporting and centralizing knowledge in extracellular vesicle research
    In: Nature Methods Vol. 14 (2017) Nr. 3, pp. 228 - 232
    Online Full Text: dx.doi.org/
  • König, Lisa; Rebmann, Vera; Hoffmann, Oliver; Bittner, A. K.; Wagner, B.; Manvailer, L. F. S.; Schramm, S.; Bankfalvi, Agnes; Giebel, Bernd; Kimmig, Rainer; Horn, Peter A.; Kasimir-Bauer, Sabine
    Breast cancer-derived extracellular vesicles : clinical and prognostic impact
    In: Cancer Research Vol. 76 (2016) Nr. 14 Suppl., pp. 3152
    Online Full Text: dx.doi.org/
  • Fais, Stefano; O'Driscoll, Lorraine; Borras, Francesc E.; Buzas, Edit; Camussi, Giovanni; Cappello, Francesco; Carvalho, Joana; Cordeiro Da Silva, Anabela; Del Portillo, Hernando; El Andaloussi, Samir; Ficko Trček, Tanja; Furlan, Roberto; Hendrix, An; Gursel, Ihsan; Kralj-Iglic, Veronika; Kaeffer, Bertrand; Kosanovic, Maja; Lekka, Marilena E.; Lipps, Georg; Logozzi, Mariantonia; Marcilla, Antonio; Sammar, Marei; Llorente, Alicia; Nazarenko, Irina; Oliveira, Carla; Pocsfalvi, Gabriella; Rajendran, Lawrence; Raposo, Graça; Rohde, Eva; Siljander, Pia; Van Niel, Guillaume; Vasconcelos, M. Helena; Yáñez-Mó, María; Yliperttula, Marjo L.; Zarovni, Natasa; Zavec, Apolonija Bedina; Giebel, Bernd
    Evidence-Based Clinical Use of Nanoscale Extracellular Vesicles in Nanomedicine
    In: ACS Nano Vol. 10 (2016) Nr. 4, pp. 3886 - 3899
    Online Full Text: dx.doi.org/
  • Hönes, J M; Botezatu, L; Helness, A; Vadnais, C; Vassen, L; Robert, F; Hergenhan, S M; Thivakaran, A; Schütte, J; Al-Matary, Y S; Lams, R F; Fraszscak, J; Makishima, H; Radivoyevitch, T; Przychodzen, B; da Conceição Castro, S V; Görgens, A; Giebel, Bernd; Klein-Hitpaß, Ludger; Lennartz, K; Heuser, M; Thiede, C; Ehninger, G; Dührsen, Ulrich; Maciejewski, J; Möröy, T; Khandanpour, Cyrus
    GFI1 as a novel prognostic and therapeutic factor for AML/MDS
    In: Leukemia Vol. 30 (2016) Nr. 6, pp. 1237 - 1245
    Online Full Text: dx.doi.org/
  • Radtke, Stefan; Görgens, André; Kordelas, Lambros; Schmidt, Markus; Kimmig, Rainer; Köninger, Angela; Horn, Peter; Giebel, Bernd
    CD133 allows elaborated discrimination and quantification of haematopoietic progenitor subsets in human haematopoietic stem cell transplants
    In: British Journal of Haematology Vol. 169 (2015) Nr. 6, pp. 868 - 878
    Online Full Text: dx.doi.org/
  • Goergens, Andre; Ludwig, Anna-Krist; Moellmann, Michael; Krawczyk, Adalbert; Dürig, Jan; Hanenberg, Helmut; Horn, Peter A.; Giebel, Bernd
    Multipotent Hematopoietic Progenitors Divide Asymmetrically to Create Progenitors of the Lymphomyeloid and Erythromyeloid Lineages
    In: Stem Cell Reports Vol. 5 (2015) Nr. 1, pp. 154 - 155
    Online Full Text: dx.doi.org/ (Open Access)
  • Malyar, Mohammad; Radtke, Stefan; Malyar, Khalil; Arjumand, Jawed; Horn, Peter; Kröger, Knut; Freisinger, Eva; Reinecke, Holger; Giebel, Bernd; Brock, Franz-Eduard
    Autologous bone marrow mononuclear cell therapy improves symptoms in patients with end-stage peripheral arterial disease and reduces inflammation-associated parameters
    In: Cytotherapy Vol. 16 (2014) Nr. 9, pp. 1270 - 1279
    Online Full Text: dx.doi.org/
  • Hemeda, Hatim; Giebel, Bernd; Wagner, Wolfgang
    Evaluation of human platelet lysate versus fetal bovine serum for culture of mesenchymal stromal cells
    In: Cytotherapy Vol. 16 (2014) Nr. 2, pp. 170 - 180
    Online Full Text: dx.doi.org/
  • Kordelas, L; Rebmann, Vera; Ludwig, A-K; Radtke, Stefan; Ruesing, J; Döppner, Thorsten R.; Epple, Matthias; Horn, Peter; Beelen, Dietrich; Giebel, Bernd
    MSC-derived exosomes : a novel tool to treat therapy-refractory graft-versus-host disease
    In: Leukemia Vol. 28 (2014) Nr. 4, pp. 970 - 973
    Online Full Text: dx.doi.org/
  • Gorgens, A.; Ludwig, A. K.; Mollmann, M.; Krawczyk, Adalbert; Dürig, Jan; Hanenberg, H.; Horn, Peter; Giebel, Bernd
    Multipotent hematopoietic progenitors divide asymmetrically to create progenitors of the lymphomyeloid and erythromyeloid lineages
    In: Stem Cell Reports Vol. 3 (2014) Nr. 6, pp. 1058 - 1072
    Online Full Text: dx.doi.org/ (Open Access)
  • Görgens, André; Radtke, Stefan; Möllmann, Michael; Cross, Michael; Dürig, Jan; Horn, Peter; Giebel, Bernd
    Revision of the Human Hematopoietic Tree: Granulocyte Subtypes Derive from Distinct Hematopoietic Lineages
    In: Cell Reports Vol. 3 (2013) Nr. 5, pp. 1539 - 1552
    Online Full Text: dx.doi.org/
  • Weide, Benjamin; Schilling, Bastian; Paschen, Annette; Görgens, Andre; Sucker, Antje; Zhao, Fang; Giebel, Bernd; Griewank, Klaus; Schadendorf, Dirk
    Vemurafenib reverses immunosuppression by myeloid derived suppressor cells
    In: Cancer Research Vol. 73 (2013) Nr. 8 Suppl., pp. 4714
    Online Full Text: dx.doi.org/
  • Goebel, Maria; Möllmann, Michael; Gorgens, A.; Dührsen, Ulrich; Huttmann, A.; Giebel, Bernd; Unger, A.; Choidas, A.; Dürig, Jan
    Preclinical Testing of a Novel Axl-Kinase Inhibitor in Chronic Lymphocytic Leukemia
    In: Blood Vol. 120 (2012) Nr. 21,
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