ZMB Member Shirley Knauer

ZMB Member
Shirley Knauer

Next ZMB-Member
portrait Shirley Knauer
© Knauer

Prof. Dr. Shirley Knauer

Faculty of Biology
University Duisburg-Essen
45141 Essen

Research Overview

Our research interests focus on molecular and translational clinical oncology as well as on the biomedical transfer of the compiled results. They aim on a detailed understanding of the regulation of nucleo-cytoplasmic transport processes, their impact on malignant transformation in cancer development, the discovery of novel therapeutic targets as well as the establishment of novel drug-screening assays.

Current projects concentrate on the molecular and pathophysiological function of the "Inhibitor of Apoptosis" protein Survivin, which is attributed a decisive role in the development of cancer and other diseases.

Using corroborating experimental approaches including microinjection experiments, flourescence as well as confocal microscopy, we could demonstrate that Survivin is actively exported from the cell nucleus. This transport process was not only a prerequisite for protection of cancer cells against radio- and chemotherapy by Survivin, but also plays a causal role in the treatment response of cancer patients. Therefore, targeted interference with the nuclear export of survivin by molecular decoys seems to be a highly promising innovative strategy to impede growth and therapy resistance of cancer cells. Additionally, the detailed functional characterisation of the anti-apoptotic protein Survivin and its cellular transport activity led to novel groundbreaking insights concerning the essential involvement of transport receptors in mitotic processes.

Read more

Selected Publications

    Journal articles

  • Rick, Tim; Kreiling, Vanessa; Höing, Alexander; Fiedler, Svenja; Glatter, Timo; Steinchen, Wieland; Hochberg, Georg; Bähre, Heike; Seifert, Roland; Bange, Gert; Knauer, Shirley K.; Graumann, Peter L.; Thormann, Kai M.; Roland, Seifert
    GGDEF domain as spatial on-switch for a phosphodiesterase by interaction with landmark protein HubP
    In: npj Biofilms and Microbiomes Vol. 8 (2022) Nr. 1, 35
  • Hensel, Astrid; Stahl, Paul; Moews, Lisa; König, Lena; Patwardhan, Rutuja; Höing, Alexander; Schulze, Nina; Nalbant, Perihan; Stauber, Roland H.; Knauer, Shirley
    The Taspase1/Myosin1f-axis regulates filopodia dynamics
    In: iScience Vol. 25 (2022) Nr. 6, 104355
  • Meiners, Annika; Bäcker, Sandra; Hadrovic, Inesa; Heid, Christian; Beuck, Christine; Ruiz-Blanco, Yasser B.; Mieres-Perez, Joel; Pörschke, Marius; Grad, Jean-Noël; Vallet, Cecilia; Hoffmann, Daniel; Bayer, Peter; Sánchez-García, Elsa; Schrader, Thomas; Knauer, Shirley
    Specific inhibition of the Survivin–CRM1 interaction by peptide-modified molecular tweezers
    In: Nature Communications Vol. 12 (2021) Nr. 1, pp. 1505
  • Gigante, Alba; Sijbesma, Eline; Sánchez-Murcia, Pedro A.; Hu, Xiaoyu; Bier, David; Bäcker, Sandra; Knauer, Shirley; Gago, Federico; Ottmann, Christian; Schmuck, Carsten
    A Supramolecular Stabilizer of the 14-3-3ζ/ERα Protein-Protein Interaction with a Synergistic Mode of Action
    In: Angewandte Chemie International Edition Vol. 132 (2020) Nr. 13, pp. 5322 - 5325
  • Westmeier, Dana; Posselt, Gernot; Hahlbrock, Angelina; Bartfeld, Sina; Vallet, Cecilia; Abfalter, Carmen; Docter, Dominic; Knauer, Shirley; Wessler, Silja; Stauber, Roland H.
    Erratum : Nanoparticle binding attenuates the pathobiology of gastric cancer-associated: Helicobacter pylori
    In: Nanoscale Vol. 12 (2020) Nr. 3, pp. 2154 - 2155
  • Vallet, Cecilia; Aschmann, Dennis; Beuck, Christine; Killa, Matthias; Meiners, Annika; Mertel, Marcel; Ehlers, Martin; Bayer, Peter; Schmuck, Carsten; Giese, Michael R. A.; Knauer, Shirley
    Functional Disruption of the Cancer-Relevant Interaction between Survivin and Histone H3 with a Guanidiniocarbonyl Pyrrole Ligand
    In: Angewandte Chemie International Edition Vol. 59 (2020) Nr. 14, pp. 5567 - 5571
  • van den Boom, Johannes; Hensel, Astrid; Trusch, Franziska; Matena, Anja; Siemer, Svenja; Guel, Désirée; Docter, Dominic; Höing, Alexander; Bayer, Peter; Stauber, Roland H.; Knauer, Shirley
    The other side of the corona : Nanoparticles inhibit the protease taspase1 in a size-dependent manner
    In: Nanoscale Vol. 12 (2020) Nr. 37, pp. 19093 - 19103
  • Siemer, Svenja; Westmeier, Dana; Barz, Matthias; Eckrich, Jonas; Wünsch, Désirée; Seckert, Christof; Thyssen, Christian; Schilling, Oliver; Hasenberg, Mike; Pang, Chengfan; Docter, Dominic; Knauer, Shirley K.; Stauber, Roland H.; Strieth, Sebastian
    Biomolecule-corona formation confers resistance of bacteria to nanoparticle-induced killing : Implications for the design of improved nanoantibiotics
    In: Biomaterials Vol. 192 (2019) pp. 551 - 559
  • Westmeier, Dana; Hahlbrock, Angelina; Reinhardt, Christoph; Fröhlich-Nowoisky, Janine; Wessler, Silja; Vallet, Cecilia; Pöschl, Ulrich; Knauer, Shirley; Stauber, Roland H.
    Nanomaterial-microbe cross-talk : physicochemical principles and (patho)biological consequences
    In: Chemical Society Reviews Vol. 47 (2018) Nr. 14, pp. 5312 - 5337
  • Abfalter, Carmen; Docter, Dominic; Wessler, Silja; Vallet, Cecilia; Westmeier, Dana; Knauer, Shirley; Posselt, Gernot; Stauber, Roland H.; Hahlbrock, Angelina; Bartfeld, Sina
    Nanoparticle binding attenuates the pathobiology of gastric cancer-associated Helicobacter pylori
    In: Nanoscale Vol. 10 (2018) Nr. 3, pp. 1453 - 1463
  • Jana, Poulami; Samanta, Krishnananda; Bäcker, Sandra; Zellermann, Elio; Knauer, Shirley; Schmuck, Carsten
    Efficient Gene Transfection through Inhibition of β-Sheet (Amyloid Fiber) Formation of a Short Amphiphilic Peptide by Gold Nanoparticles
    In: Angewandte Chemie International Edition Vol. 56 (2017) Nr. 28, pp. 8083 - 8088
  • Jiang, Hao; Hu, Xiao-Yu; Schlesiger, Stefanie; Li, Mao; Zellermann, Elio; Knauer, Shirley; Schmuck, Carsten
    Morphology-Dependent Cell Imaging by Using a Self-Assembled Diacetylene Peptide Amphiphile
    In: Angewandte Chemie International Edition Vol. 56 (2017) Nr. 46, pp. 14526 - 14530
  • Li, Mao; Ehlers, Martin; Schlesiger, Stefanie; Zellermann, Elio; Knauer, Shirley; Schmuck, Carsten
    Incorporation of a Non-Natural Arginine Analogue into a Cyclic Peptide Leads to Formation of Positively Charged Nanofibers Capable of Gene Transfection
    In: Angewandte Chemie International Edition Vol. 55 (2016) Nr. 2, pp. 598 - 601
  • Li, Mao; Schlesiger, Stefanie; Knauer, Shirley; Schmuck, Carsten
    A Tailor-Made Specific Anion-Binding Motif in the Side Chain Transforms a Tetrapeptide into an Efficient Vector for Gene Delivery
    In: Angewandte Chemie International Edition Vol. 54 (2015) Nr. 10, pp. 2941 - 2944
  • Docter, Dominic; Westmeier, Dana; Markiewicz, Marta; Stolte, Stefan; Knauer, Shirley; Stauber, Roland H.
    The nanoparticle biomolecule corona : lessons learned – challenge accepted?
    In: Chemical Society Reviews Vol. 44 (2015) Nr. 17, pp. 6094 - 6121
  • Docter, Dominic; Distler, Ute; Storck, Wiebke; Kuharev, Jörg; Wünsch, Desirée; Hahlbrock, Angelina; Knauer, Shirley K.; Tenzer, Stefan; Stauber, Roland H.
    Quantitative profiling of the protein coronas that form around nanoparticles
    In: Nature Protocols Vol. 9 (2014) Nr. 9, pp. 2030 - 2044
  • Tenzer, Stefan; Docter, Dominic; Kuharev, Jörg; Musyanovych, Anna; Fetz, Verena; Hecht, Rouven; Schlenk, Florian; Fischer, Dagmar; Kiouptsi, Klytaimnistra; Reinhardt, Christoph; Landfester, Katharina; Schild, Hansjörg; Maskos, Michael; Knauer, Shirley; Stauber, Roland H.
    Rapid formation of plasma protein corona critically affects nanoparticle pathophysiology
    In: Nature Nanotechnology Vol. 8 (2013) Nr. 10, pp. 772 - 781
  • Krämer, Oliver H.; Stauber, Roland H.; Bug, Gesine; Hartkamp, Jörg; Knauer, Shirley
    SIAH proteins : Critical roles in leukemogenesis
    In: Leukemia Vol. 27 (2013) Nr. 4, pp. 792 - 802
  • Kuchelmeister, Hannes Y.; Karczewski, Sarah; Gutschmidt, Aljona; Knauer, Shirley; Schmuck, Carsten
    Utilizing Combinatorial Chemistry and Rational Design : Peptidic Tweezers with Nanomolar Affinity to DNA Can Be Transformed into Efficient Vectors for Gene Delivery by Addition of a Lipophilic Tail
    In: Angewandte Chemie International Edition Vol. 52 (2013) Nr. 52, pp. 14016 - 14020
  • Pless, Birgit; Oehm, Clarissa; Knauer, Shirley; Stauber, Roland; Dingermann, Theo; Marschalek, Rolf
    The heterodimerization domains of MLL-FYRN and FYRC-are potential target structures in t(4;11) leukemia.
    In: Leukemia Vol. 25 (2011) Nr. 4, pp. 663 - 670
  • Knauer, Shirley; Bier, Carolin; Habtemichael, Negusse; Stauber, Roland H.
    The Survivin-Crm1 interaction is essential for chromosomal passenger complex localization and function.
    In: EMBO Reports Vol. 7 (2006) Nr. 12, pp. 1259 - 1265